Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cytochrome c1 CRISPR/Cas9 KO Plasmid (m): sc-426027
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cytochrome c1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cytochrome c1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cytochrome c1 Antibody (A-5): sc-514435
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cytochrome c1 CRISPR/Cas9 KO Plasmid (m) | sc-426027 | 20 µg | $397.00 |
Overview
Mouse Cyc1 encodes cytochrome c1, an essential subunit of mitochondrial complex III (ubiquinol–cytochrome c reductase) that mediates electron transfer from ubiquinol to cytochrome c within the inner mitochondrial membrane. Through its role in the Q cycle, cytochrome c1 contributes to proton motive force generation that powers ATP synthesis and supports redox homeostasis. Disruption of complex III components can impair oxidative phosphorylation, elevate reactive oxygen species, and trigger secondary effects on apoptosis signaling and mitochondrial quality control pathways such as mitophagy. Cyc1 is therefore widely studied in models of mitochondrial dysfunction and metabolic stress that intersect with neuromuscular and neurodegenerative disease mechanisms.
cytochrome c1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cyc1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cyc1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cyc1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cytochrome c1 protein expression.
This CRISPR knockout system enables efficient generation of Cyc1-deficient cell models for investigation of cytochrome c1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cyc1 exon(s) critical for cytochrome c1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cyc1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cytochrome c1 CRISPR/Cas9 KO Plasmid (m) and cytochrome c1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cyc1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cytochrome c1 HDR Plasmid (m) and cytochrome c1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cyc1 homology arms to support homology-directed repair at defined Cyc1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.