Cypher Antibody (E-8): sc-374359
- Cypher Antibody (E-8) is a mouse monoclonal IgG2a κ Cypher antibody provided at 200 µg/ml
- raised against amino acids 572-654 mapping within an internal region of Cypher of human origin
- Cypher Antibody (E-8) is recommended for detection of Cypher of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Cypher Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cypher Antibody (E-8). This work is in progress.
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SEE ALSO...
Cypher Antibody (E-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cypher protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cypher (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cypher protein, also known as LDB3 (LIM domain binding 3), ZASP (Z-band alternatively spliced PDZ-motif protein), ORACLE, and PDLIM6 (PDZ and LIM domain 6), is a 727 amino acid protein primarily expressed in skeletal muscle, with lower expression levels in brain, placenta, and heart. Cypher protein is localized to the perinuclear region of the cytoplasm and features three LIM zinc-binding domains, which are crucial for its role as an adaptor protein. These LIM domains facilitate the coupling of protein kinase C-mediated signaling in striated muscle to the cytoskeleton, thereby playing a vital role in maintaining muscle integrity and function. Defects in the gene encoding Cypher have been linked to several forms of dilated cardiomyopathy and myofibrillar myopathy, conditions characterized by impaired cardiac muscle structure and function. Six isoforms of Cypher exist due to alternative splicing, highlighting the complexity and regulatory potential of this protein in muscle biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Cypher Antibody (E-8) References:
- Cypher, a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C. | Zhou, Q., et al. 1999. J Biol Chem. 274: 19807-13. PMID: 10391924
- Oracle, a novel PDZ-LIM domain protein expressed in heart and skeletal muscle. | Passier, R., et al. 2000. Mech Dev. 92: 277-84. PMID: 10727866
- A Cypher/ZASP mutation associated with dilated cardiomyopathy alters the binding affinity to protein kinase C. | Arimura, T., et al. 2004. J Biol Chem. 279: 6746-52. PMID: 14660611
- Genetic analysis in patients with left ventricular noncompaction and evidence for genetic heterogeneity. | Xing, Y., et al. 2006. Mol Genet Metab. 88: 71-7. PMID: 16427346
- Zasp/Cypher internal ZM-motif containing fragments are sufficient to co-localize with alpha-actinin--analysis of patient mutations. | Klaavuniemi, T. and Ylänne, J. 2006. Exp Cell Res. 312: 1299-311. PMID: 16476425
- Zebrafish cypher is important for somite formation and heart development. | van der Meer, DL., et al. 2006. Dev Biol. 299: 356-72. PMID: 16982050
- Insights into the molecular evolution of the PDZ/LIM family and identification of a novel conserved protein motif. | Te Velthuis, AJ., et al. 2007. PLoS One. 2: e189. PMID: 17285143
- Zaspopathy in a large classic late-onset distal myopathy family. | Griggs, R., et al. 2007. Brain. 130: 1477-84. PMID: 17337483
- Barth syndrome associated with compound hemizygosity and heterozygosity of the TAZ and LDB3 genes. | Marziliano, N., et al. 2007. Am J Med Genet A. 143A: 907-15. PMID: 17394203
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cypher Antibody (E-8) | sc-374359 | 200 µg/ml | $322.00 | |||
Cypher Antibody (E-8) AC | sc-374359 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Cypher Antibody (E-8) HRP | sc-374359 HRP | 200 µg/ml | $322.00 | |||
Cypher Antibody (E-8) FITC | sc-374359 FITC | 200 µg/ml | $336.00 | |||
Cypher Antibody (E-8) PE | sc-374359 PE | 200 µg/ml | $349.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 488 | sc-374359 AF488 | 200 µg/ml | $364.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 546 | sc-374359 AF546 | 200 µg/ml | $364.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 594 | sc-374359 AF594 | 200 µg/ml | $364.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 647 | sc-374359 AF647 | 200 µg/ml | $364.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 680 | sc-374359 AF680 | 200 µg/ml | $364.00 | |||
Cypher Antibody (E-8) Alexa Fluor® 790 | sc-374359 AF790 | 200 µg/ml | $364.00 |