CYP11B2 CRISPR/Cas9 KO Plasmid (m): sc-419894
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYP11B2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CYP11B2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP11B2 CRISPR/Cas9 KO Plasmid (m) | sc-419894 | 20 µg | $397.00 |
Overview
Cyp11b2 encodes CYP11B2 (aldosterone synthase), a mitochondrial cytochrome P450 enzyme that catalyzes the terminal steps of aldosterone biosynthesis from deoxycorticosterone. This activity lies within the steroidogenic pathway, coupling electron transfer from adrenodoxin/adrenodoxin reductase to oxidative reactions that regulate mineralocorticoid output and downstream electrolyte and fluid homeostasis. In mouse adrenal zona glomerulosa cells, CYP11B2 integrates hormonal signaling cues, including calcium-dependent and angiotensin II–responsive programs, to tune steroid production. Dysregulated Cyp11b2 expression or aldosterone excess is linked to maladaptive cardiovascular and renal physiology, making the gene relevant for mechanistic studies of endocrine control and stress-responsive metabolic remodeling.
CYP11B2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cyp11b2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cyp11b2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cyp11b2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CYP11B2 protein expression.
This CRISPR knockout system enables efficient generation of Cyp11b2-deficient cell models for investigation of CYP11B2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cyp11b2 exon(s) critical for CYP11B2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cyp11b2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CYP11B2 CRISPR/Cas9 KO Plasmid (m) and CYP11B2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cyp11b2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CYP11B2 HDR Plasmid (m) and CYP11B2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cyp11b2 homology arms to support homology-directed repair at defined Cyp11b2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.