cyclin T2a/b Double Nickase Plasmid (h): sc-404586-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin T2a/b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cyclin T2a/b Double Nickase Plasmid (h) and cyclin T2a/b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCNT2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin T2a/b Antibody (2128C1a): sc-81243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin T2a/b Double Nickase Plasmid (h) | sc-404586-NIC | 20 µg | $410.00 | |||
cyclin T2a/b Double Nickase Plasmid (h2) | sc-404586-NIC-2 | 20 µg | $410.00 |
CCNT2 encodes cyclin T2 (isoforms T2a and T2b), a regulatory cyclin that partners with CDK9 to form the positive transcription elongation factor b (P-TEFb) complex. P-TEFb phosphorylates the C-terminal domain of RNA polymerase II and negative elongation factors, promoting productive transcriptional elongation and coordinating expression programs linked to cell-cycle progression, differentiation, and stress responses. Through interactions with transcriptional regulators and elongation control networks, cyclin T2 influences chromatin-associated transcription dynamics across diverse gene sets. Dysregulation of P-TEFb signaling and elongation control has been associated with altered proliferative and immune transcriptional states, making CCNT2 a useful target for mechanistic studies of transcription-dependent phenotypes.
cyclin T2a/b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCNT2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCNT2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCNT2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCNT2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.