CUG-BP1 Antibody (3B1): sc-20003

CUG-BP1 Antibody (3B1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CUG-BP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). CUG-BP1 monoclonal antibody (3B1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. CUG-BP1 plays a crucial role in the regulation of RNA splicing and translation, particularly in the context of myotonic dystrophy, an autosomal dominant neuromuscular disorder linked to a (CTG)n repeat expansion in the 3′-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 binds specifically to (CUG)8 oligonucleotides, and binding activity is significantly altered in myotonic dystrophy cells, where the nuclear binding activity of CUG-BP2 increases. This shift in binding dynamics is critical as CUG-BP1 regulates the translation of the C/EBPb transcription factor, which is essential for cell cycle regulation. In normal cells, CUG-BP1 enhances the translation of the p21 protein, promoting differentiation by binding to a GC-rich sequence in the 5′ region of p21 mRNA. However, in myotonic dystrophy cells, the reduced accumulation of CUG-BP1 leads to decreased levels of p21 and disrupts the expression of other proteins involved in cell cycle withdrawal, highlighting CUG-BP1′s importance in maintaining cellular homeostasis and potential as a therapeutic target in neuromuscular diseases.

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CUG-BP1 Antibody (3B1) References:

1. CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.  |  Timchenko, NA., et al. 1999. Nucleic Acids Res. 27: 4517-25. PMID: 10536163
2. The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system.  |  Takahashi, N., et al. 2000. Biochem Biophys Res Commun. 277: 518-23. PMID: 11032753
3. RNA CUG repeats sequester CUGBP1 and alter protein levels and activity of CUGBP1.  |  Timchenko, NA., et al. 2001. J Biol Chem. 276: 7820-6. PMID: 11124939
4. Molecular basis for impaired muscle differentiation in myotonic dystrophy.  |  Timchenko, NA., et al. 2001. Mol Cell Biol. 21: 6927-38. PMID: 11564876
5. Inactivation of CUG-BP1/CELF1 causes growth, viability, and spermatogenesis defects in mice.  |  Kress, C., et al. 2007. Mol Cell Biol. 27: 1146-57. PMID: 17130239
6. The role of CUGBP1 in age-dependent changes of liver functions.  |  Jones, K., et al. 2012. Ageing Res Rev. 11: 442-9. PMID: 22446383
7. RNA Binding Protein CUGBP1 Inhibits Liver Cancer in a Phosphorylation-Dependent Manner.  |  Lewis, K., et al. 2017. Mol Cell Biol. 37: PMID: 28559429
8. CUGBP1, a crucial factor for heart regeneration in mice.  |  Liu, Y., et al. 2022. Cell Death Dis. 13: 120. PMID: 35136022
9. Small molecule targeting CELF1 RNA-binding activity to control HSC activation and liver fibrosis.  |  Tan, Y., et al. 2022. Nucleic Acids Res. 50: 2440-2451. PMID: 35234905
10. Glycyrrhizic acid alleviates liver fibrosis in vitro and in vivo via activating CUGBP1-mediated IFN-γ/STAT1/Smad7 pathway.  |  Guo, M., et al. 2023. Phytomedicine. 112: 154587. PMID: 36805480
11. High-Throughput Transcriptomics of Celf1 Conditional Knockout Lens Identifies Downstream Networks Linked to Cataract Pathology.  |  Siddam, AD., et al. 2023. Cells. 12: PMID: 37048143
12. Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.  |  Timchenko, LT., et al. 1996. Nucleic Acids Res. 24: 4407-14. PMID: 8948631

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CUG-BP1 Antibody (3B1) has 98 citations in a variety of scientific publications.

Primary antibodies like Anti-CUG-BP1 Antibody (3B1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CUG-BP1 Antibody (3B1) Specifications

Alternate Product Name: CELF1

Product Clone Name: 3B1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CUG-BP1

Epitope Species: human

Product Reactivity/Detection: CUG-BP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 98 citations in various scientific publications

Available Conjugates/Forms for CUG-BP1 Antibody (3B1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin