Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTAGE5 CRISPR/Cas9 KO Plasmid (h): sc-406164
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTAGE5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTAGE5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTAGE5 CRISPR/Cas9 KO Plasmid (h) | sc-406164 | 20 µg | $397.00 |
Overview
CTAGE5 (also known as MIA2) encodes a membrane-associated component of the ER export machinery that supports COPII-dependent trafficking and organization of ER exit sites. By coordinating cargo loading and vesicle formation, CTAGE5 contributes to efficient secretion and maintenance of ER proteostasis, processes tightly linked to the unfolded protein response and ER stress signaling. Disruption of CTAGE5 function can alter secretory pathway flux, impact intracellular protein handling, and reshape cellular responses to metabolic or inflammatory stress. In human biology, these pathways are frequently investigated in contexts where secretory capacity and ER homeostasis influence disease-relevant phenotypes, including tumor cell adaptation and immune-related signaling.
CTAGE5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MIA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MIA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MIA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTAGE5 protein expression.
This CRISPR knockout system enables efficient generation of MIA2-deficient cell models for investigation of CTAGE5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MIA2 exon(s) critical for CTAGE5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MIA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTAGE5 CRISPR/Cas9 KO Plasmid (h) and CTAGE5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MIA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTAGE5 HDR Plasmid (h) and CTAGE5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MIA2 homology arms to support homology-directed repair at defined MIA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.