Crk II Double Nickase Plasmid (h): sc-402415-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Crk II Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Crk II Double Nickase Plasmid (h) and Crk II Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRK. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Crk II Antibody (B-4): sc-390132
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Crk II Double Nickase Plasmid (h) | sc-402415-NIC | 20 µg | $410.00 | |||
Crk II Double Nickase Plasmid (h2) | sc-402415-NIC-2 | 20 µg | $410.00 |
CRK encodes Crk II, an SH2/SH3 domain–containing adaptor protein that couples phosphorylated tyrosine motifs to downstream effectors controlling signal transduction. Crk II participates in integrin and growth factor receptor pathways by linking proteins such as p130CAS/BCAR1 and DOCK-family GEFs to Rho GTPase signaling, thereby regulating actin remodeling, cell adhesion, migration, and survival. Through its roles in focal adhesion dynamics and MAPK/PI3K-associated networks, altered CRK signaling is frequently studied in contexts of oncogenic transformation, invasion, and metastasis-associated phenotypes. Dysregulation of Crk II–mediated complexes has also been investigated in models of aberrant developmental signaling and stress-response pathways relevant to human disease biology.
Crk II Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRK locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRK. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRK function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRK-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.