Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRISP-3 CRISPR/Cas9 KO Plasmid (m): sc-419036
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRISP-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CRISP-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRISP-3 Antibody (D-6): sc-377505
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRISP-3 CRISPR/Cas9 KO Plasmid (m) | sc-419036 | 20 µg | $397.00 |
Overview
Crisp3 encodes cysteine-rich secretory protein 3 (CRISP-3), a secreted CAP superfamily member implicated in extracellular signaling and modulation of immune and epithelial cell interactions. CRISP-3 is enriched in exocrine and mucosal tissues and has been linked to regulation of protease activity, antimicrobial defense, and inflammatory cues within the local microenvironment. Through its extracellular localization, CRISP-3 can influence tissue remodeling and innate immune processes that intersect with wound response and barrier function pathways. Dysregulated CRISP-3 expression has been reported in inflammation-associated contexts and cancer-related transcriptomic signatures, making Crisp3 a useful node for studying microenvironmental regulation and secreted factor biology in mouse models.
CRISP-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Crisp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Crisp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Crisp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CRISP-3 protein expression.
This CRISPR knockout system enables efficient generation of Crisp3-deficient cell models for investigation of CRISP-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Crisp3 exon(s) critical for CRISP-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Crisp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CRISP-3 CRISPR/Cas9 KO Plasmid (m) and CRISP-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Crisp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CRISP-3 HDR Plasmid (m) and CRISP-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Crisp3 homology arms to support homology-directed repair at defined Crisp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.