Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
connexin 40 Double Nickase Plasmid (h): sc-401031-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- connexin 40 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- connexin 40 Double Nickase Plasmid (h) and connexin 40 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GJA5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: connexin 40 Antibody (B-3): sc-365107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
connexin 40 Double Nickase Plasmid (h) | sc-401031-NIC | 20 µg | $410.00 | |||
connexin 40 Double Nickase Plasmid (h2) | sc-401031-NIC-2 | 20 µg | $410.00 |
GJA5 encodes connexin 40 (Cx40), a gap junction channel subunit that forms intercellular conduits for electrical and metabolic coupling through connexons in the plasma membrane. In human tissues, Cx40 is prominent in vascular endothelium and the cardiac conduction system, where it supports coordinated propagation of signals and contributes to tissue homeostasis. Connexin 40 participates in gap junction assembly/turnover, cell–cell communication, and regulation of vascular tone and conduction velocity. Dysregulated GJA5 expression or connexin channel function has been associated with cardiovascular phenotypes including atrial conduction abnormalities and arrhythmia susceptibility, making it relevant for mechanistic studies of electrophysiology and endothelial signaling.
connexin 40 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GJA5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GJA5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GJA5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GJA5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.