COG4 CRISPR/Cas9 KO Plasmid (h): sc-416653
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COG4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COG4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COG4 CRISPR/Cas9 KO Plasmid (h) | sc-416653 | 20 µg | $397.00 |
Overview
COG4 encodes a core subunit of the conserved oligomeric Golgi (COG) tethering complex that maintains Golgi architecture and coordinates retrograde vesicle trafficking within the Golgi stack. By regulating vesicle docking and SNARE-dependent fusion, COG4 supports glycosylation enzyme recycling and proper processing of secretory and membrane proteins across the endomembrane system. Disruption of COG complex function perturbs Golgi-to-ER and intra-Golgi transport, leading to altered protein glycosylation and broad effects on secretion, receptor maturation, and membrane composition. Variants in COG4 and related COG components are associated with congenital disorders of glycosylation and neurodevelopmental phenotypes, making this gene relevant for studying Golgi trafficking-linked disease mechanisms.
COG4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COG4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COG4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COG4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COG4 protein expression.
This CRISPR knockout system enables efficient generation of COG4-deficient cell models for investigation of COG4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COG4 exon(s) critical for COG4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COG4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COG4 CRISPR/Cas9 KO Plasmid (h) and COG4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COG4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COG4 HDR Plasmid (h) and COG4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COG4 homology arms to support homology-directed repair at defined COG4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.