CNOT10 Antibody (H-9): sc-514570
- CNOT10 Antibody (H-9) is a mouse monoclonal IgG1 κ CNOT10 antibody provided at 200 µg/ml
- raised against amino acids 302-468 mapping within an internal region of CNOT10 of human origin
- CNOT10 Antibody (H-9) is recommended for detection of CNOT10 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CNOT10 Antibody (H-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CNOT10 Antibody (H-9) for WB applications. These reagents are now offered in bundles with CNOT10 Antibody (H-9) (see ordering information below).
CNOT10 Antibody (H-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects CNOT10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CNOT10 (H-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CNOT10 protein is a crucial subunit of the CCR4-NOT complex, which is composed of at least five other CNOT subunits and TAB182. This complex plays a vital role in regulating gene expression and mRNA degradation, making CNOT10 essential for maintaining cellular homeostasis. CNOT10 protein is involved in the regulation of histone modifications, particularly influencing the methylation of histone H3 at lysine 4 through a ubiquitin-dependent pathway that likely engages the proteasome. This post-translational modification is significant as CNOT10 can alter chromatin structure and gene accessibility, thereby impacting transcriptional regulation and cellular responses to various stimuli. The CCR4-NOT complex′s function as a transcriptional regulator and repressor of nuclear receptor signaling is particularly relevant in the context of cancer, where dysregulation of these pathways can lead to tumorigenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CNOT10 Antibody (H-9) References:
- DNA damage and replication stress induced transcription of RNR genes is dependent on the Ccr4-Not complex. | Mulder, KW., et al. 2005. Nucleic Acids Res. 33: 6384-92. PMID: 16275785
- Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription. | Winkler, GS., et al. 2006. EMBO J. 25: 3089-99. PMID: 16778766
- mRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes. | Behm-Ansmant, I., et al. 2006. Genes Dev. 20: 1885-98. PMID: 16815998
- An unconventional human Ccr4-Caf1 deadenylase complex in nuclear cajal bodies. | Wagner, E., et al. 2007. Mol Cell Biol. 27: 1686-95. PMID: 17178830
- CCR4/NOT complex associates with the proteasome and regulates histone methylation. | Laribee, RN., et al. 2007. Proc Natl Acad Sci U S A. 104: 5836-41. PMID: 17389396
- Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex. | Mulder, KW., et al. 2007. Nucleic Acids Res. 35: 2428-39. PMID: 17392337
- A SAGA-independent function of SPT3 mediates transcriptional deregulation in a mutant of the Ccr4-not complex in Saccharomyces cerevisiae. | James, N., et al. 2007. Genetics. 177: 123-35. PMID: 17660549
- The Ccr4-not complex regulates Skn7 through Srb10 kinase. | Lenssen, E., et al. 2007. Eukaryot Cell. 6: 2251-9. PMID: 17965252
- Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1. | Garapaty, S., et al. 2008. J Biol Chem. 283: 6806-16. PMID: 18180299
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNOT10 Antibody (H-9) | sc-514570 | 200 µg/ml | $322.00 | |||
CNOT10 Antibody (H-9): m-IgG Fc BP-HRP Bundle | sc-527916 | 200 µg Ab; 10 µg BP | $361.00 | |||
CNOT10 Antibody (H-9): m-IgG1 BP-HRP Bundle | sc-533289 | 200 µg Ab; 20 µg BP | $361.00 | |||
CNOT10 Antibody (H-9) AC | sc-514570 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CNOT10 Antibody (H-9) HRP | sc-514570 HRP | 200 µg/ml | $322.00 | |||
CNOT10 Antibody (H-9) FITC | sc-514570 FITC | 200 µg/ml | $336.00 | |||
CNOT10 Antibody (H-9) PE | sc-514570 PE | 200 µg/ml | $349.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 488 | sc-514570 AF488 | 200 µg/ml | $364.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 546 | sc-514570 AF546 | 200 µg/ml | $364.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 594 | sc-514570 AF594 | 200 µg/ml | $364.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 647 | sc-514570 AF647 | 200 µg/ml | $364.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 680 | sc-514570 AF680 | 200 µg/ml | $364.00 | |||
CNOT10 Antibody (H-9) Alexa Fluor® 790 | sc-514570 AF790 | 200 µg/ml | $364.00 |