Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CKR-10 CRISPR/Cas9 KO Plasmid (h): sc-407896
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CKR-10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CKR-10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CKR-10 Antibody (E-2): sc-365957
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CKR-10 CRISPR/Cas9 KO Plasmid (h) | sc-407896 | 20 µg | $397.00 |
Overview
CCR10 encodes the chemokine receptor CKR-10, a seven-transmembrane GPCR that binds the ligands CCL27 and CCL28 to guide leukocyte chemotaxis and tissue-specific homing. CKR-10 signaling engages heterotrimeric G proteins to regulate calcium flux, MAPK and PI3K-dependent pathways, integrin activation, and cytoskeletal remodeling that collectively shape cell migration and positioning at epithelial barriers. This receptor is implicated in immune surveillance and inflammatory trafficking programs in skin and mucosal tissues, where altered CCR10 activity can shift local immune cell composition. Dysregulated CCR10-associated chemokine networks have been studied in the context of chronic inflammation and tumor microenvironment biology, supporting its relevance as a mechanistic node for migration-focused research.
CKR-10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCR10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCR10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCR10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CKR-10 protein expression.
This CRISPR knockout system enables efficient generation of CCR10-deficient cell models for investigation of CKR-10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCR10 exon(s) critical for CKR-10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCR10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CKR-10 CRISPR/Cas9 KO Plasmid (h) and CKR-10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCR10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CKR-10 HDR Plasmid (h) and CKR-10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCR10 homology arms to support homology-directed repair at defined CCR10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.