CHD8 CRISPR/Cas9 KO Plasmid (m): sc-426734
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHD8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHD8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHD8 CRISPR/Cas9 KO Plasmid (m) | sc-426734 | 20 µg | $397.00 |
Overview
Chd8 encodes chromodomain helicase DNA-binding protein 8 (CHD8), an ATP-dependent chromatin remodeler that regulates nucleosome positioning and transcriptional programs during development. CHD8 influences enhancer and promoter accessibility, linking chromatin architecture to cell-cycle control, neuronal differentiation, and Wnt/β-catenin–associated transcriptional outputs. In mouse systems, Chd8 is widely used to interrogate mechanisms of neurodevelopment and gene regulatory networks, as perturbation of CHD8 dosage has been connected to autism spectrum disorder–associated biology in genetic and functional studies. These properties make CHD8 a key node for studying chromatin-mediated control of lineage specification and transcriptional homeostasis.
CHD8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chd8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chd8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chd8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHD8 protein expression.
This CRISPR knockout system enables efficient generation of Chd8-deficient cell models for investigation of CHD8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chd8 exon(s) critical for CHD8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chd8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHD8 CRISPR/Cas9 KO Plasmid (m) and CHD8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chd8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHD8 HDR Plasmid (m) and CHD8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chd8 homology arms to support homology-directed repair at defined Chd8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.