Cdc123 Antibody (G-10) recommended for detection of Cdc123 of mouse, rat and human origin by WB, IP, IF and ELISA

Cdc123 Antibody (G-10): sc-365596

Cdc123 Antibody (G-10) is rated 5.0 out of 5 by 2.
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Datasheets

    • Cdc123 Antibody (G-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Cdc123 of human origin
    • recommended for detection of Cdc123 of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with Cdc123 (G-10): sc-365596 monoclonal antibody?

Asked by: cjMara
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Positive immunofluorescence staining of methanol Positive immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization. -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Signal via western blot analysis of Cdc123 Signal via western blot analysis of Cdc123 expression in AML-193 whole cell lysate. -SCBT QC
Date published: 2015-01-09
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