CD73 (h2): 293T Lysate: sc-117055

CD73 (h2): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line modified to express the SV40 large T antigen, enhancing its transfection efficiency and protein production capabilities. This lysate is enriched with human CD73, also known as ecto-5'-nucleotidase, a glycosylphosphatidylinositol (GPI)-anchored cell surface enzyme. CD73 plays a crucial role in the extracellular adenosine pathway by catalyzing the conversion of extracellular AMP to adenosine, which acts as an important signaling molecule involved in various physiological processes including immune response modulation, inflammation, and tissue protection. In research, CD73 (h2): 293T Lysate is used to study the enzymatic activity and regulatory mechanisms of CD73. Researchers utilize this lysate in Western blotting to detect and quantify CD73 expression levels under different experimental conditions, providing insights into its regulation and function. The lysate is also employed in enzyme activity assays to measure the conversion of AMP to adenosine, helping to explain the role of CD73 in purinergic signaling pathways. Additionally, CD73 (h2): 293T Lysate is valuable in co-immunoprecipitation assays to identify protein-protein interactions involving CD73, which helps to understand its integration within larger signaling complexes. Flow cytometry and binding studies are also conducted using this lysate to explore CD73 interactions with its ligands and to assess its role in cellular adhesion and signaling processes.