Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h): sc-400310-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h) and CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TFRC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1): sc-32272
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h) | sc-400310-NIC | 20 µg | $410.00 | |||
CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h2) | sc-400310-NIC-2 | 20 µg | $410.00 |
TFRC (CD71) encodes the transferrin receptor, a type II membrane glycoprotein that mediates clathrin-dependent endocytosis of transferrin-bound iron and supports cellular iron homeostasis. Receptor trafficking and recycling are coupled to endosomal acidification, iron release, and coordination of iron-dependent processes including mitochondrial respiration, heme synthesis, and ribonucleotide reductase activity for DNA synthesis. TFRC expression is tightly regulated by iron-responsive element/iron regulatory protein signaling and often correlates with proliferative state due to elevated iron demand. Dysregulated TFRC-linked iron handling is studied in contexts such as anemia, neurodegeneration, infection biology, and tumor cell metabolism where altered iron uptake can influence oxidative stress and growth programs.
CD71/TFRC/Transferrin Receptor Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TFRC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TFRC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TFRC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TFRC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.