CD40 Antibody (LOB-11) recommended for detection of CD40 of human origin by WB, IP, IF, IHC(P) and FCM

CD40 Antibody (LOB-11): sc-13528

CD40 Antibody (LOB-11) is rated 4.0 out of 5 by 8.
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Datasheets

    • CD40 Antibody (LOB-11) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against the extracellular domain of CD40 of human origin
    • CD40 Antibody (LOB-11) is recommended for detection of CD40 of human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD40 Antibody (LOB-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD40 (LOB-11): sc-13528.

Can CD40 (LOB-11): sc-13528 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD40 (LOB-11): sc-13528 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Very good Very good antibody. It works in WB of whole cell lysates of human cells. 1/250 dilution.
Date published: 2020-06-10
Rated 1 out of 5 by from dose not work does not work at all on PFA-fixed paraffin-embeded human retina sections, unlike the CD40(H-10), these two are tested under same conditions.
Date published: 2018-02-15
Rated 5 out of 5 by from Publishable Western blot results using M12 Publishable Western blot results using M12.4.1. -SCBT Publication Review
Date published: 2015-06-10
Rated 5 out of 5 by from Published good quality flow cytometry data Published good quality flow cytometry data in human PC-3M and PC-3S. -SCBT Publication Review
Date published: 2015-06-07
Rated 4 out of 5 by from Robust WB signal obtained using BJAB whole Robust WB signal obtained using BJAB whole cell lysate. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Produced great FCM data for GA Produced great FCM data for GA-10 cells. -SCBT QC
Date published: 2015-04-21
Rated 4 out of 5 by from Robust immunoperoxidase membrane staining Robust immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human lymphoma. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Publishable data in WB in mouse BLCLs Publishable data in WB in mouse BLCLs. -SCBT Publication Review
Date published: 2015-01-30
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