CBX8 Antibody (C-3): sc-374332
- CBX8 Antibody (C-3) is a mouse monoclonal IgG2b κ, cited in 9 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 231-269 within an internal region of CBX8 of human origin
- Anti-CBX8 Antibody (C-3) is recommended for detection of CBX8 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CBX8 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CBX8 Antibody (C-3) for WB applications. These reagents are now offered in bundles with CBX8 Antibody (C-3) (see ordering information below).
CBX8 Antibody (C-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects CBX8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CBX8 (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CBX8, a member of the Polycomb group (PcG) proteins, plays a crucial role in gene silencing and Hox gene regulation by modifying chromatin structure during transcription, which maintains cellular identity and regulates developmental processes. CBX8, along with CBX4, exhibits structural similarities to M33 and interacts with PcG protein RING1 through a conserved c-box motif at RING1′s C-terminus. CBX8 binds to covalently modified forms of RING1 in vivo, highlighting specific functional interactions. CBX8 also interacts with AF9′s carboxy-terminus, a transcriptional activator linked to acute leukemias, emphasizing CBX8′s significance in transcriptional regulation. The human MPc3 gene, encoding the 389 amino acid CBX8 protein, is located on chromosome 17q25.3, underscoring CBX8′s relevance in genetic studies and potential implications in cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CBX8 Antibody (C-3) References:
- RYBP, a new repressor protein that interacts with components of the mammalian Polycomb complex, and with the transcription factor YY1. | García, E., et al. 1999. EMBO J. 18: 3404-18. PMID: 10369680
- HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties. | Bárdos, JI., et al. 2000. J Biol Chem. 275: 28785-92. PMID: 10825164
- Altered retinoic acid sensitivity and temporal expression of Hox genes in polycomb-M33-deficient mice. | Bel-Vialar, S., et al. 2000. Dev Biol. 224: 238-49. PMID: 10926763
- The polycomb protein MPc3 interacts with AF9, an MLL fusion partner in t(9;11)(p22;q23) acute leukemias. | Hemenway, CS., et al. 2001. Oncogene. 20: 3798-805. PMID: 11439343
- CBX8 Exhibits Oncogenic Activity via AKT/β-Catenin Activation in Hepatocellular Carcinoma. | Zhang, CZ., et al. 2018. Cancer Res. 78: 51-63. PMID: 29066512
- CBX8 exhibits oncogenic properties and serves as a prognostic factor in hepatocellular carcinoma. | Tang, B., et al. 2019. Cell Death Dis. 10: 52. PMID: 30718464
- Upregulated CBX8 Promotes Cancer Metastasis via the WNK2/MMP2 Pathway. | Jia, Y., et al. 2020. Mol Ther Oncolytics. 19: 188-196. PMID: 33251331
- CBX8 interacts with chromatin PTEN and is involved in regulating mitotic progression. | Choi, BH., et al. 2021. Cell Prolif. 54: e13110. PMID: 34592789
- Reprogramming CBX8-PRC1 function with a positive allosteric modulator. | Suh, JL., et al. 2022. Cell Chem Biol. 29: 555-571.e11. PMID: 34715055
- CBX8 Promotes Cell Proliferation and Metastasis and Leads to Radiotherapy Tolerance of Glioma Cells. | Liu, Q., et al. 2023. Turk Neurosurg. 33: 46-52. PMID: 35713250
- TRIM28 recruits E2F1 to regulate CBX8-mediated cell proliferation and tumor metastasis of ovarian cancer. | Zhang, F., et al. 2023. Hum Cell. 36: 2113-2128. PMID: 37709991
- CBX8 promotes lung adenocarcinoma growth and metastasis through transcriptional repression of CDKN2C and SCEL. | Chen, H., et al. 2023. J Cell Physiol. 238: 2710-2723. PMID: 37733753
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBX8 Antibody (C-3) | sc-374332 | 200 µg/ml | $322.00 | |||
CBX8 Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-522702 | 200 µg Ab, 40 µg BP | $361.00 | |||
CBX8 Antibody (C-3): m-IgG2b BP-HRP Bundle | sc-549057 | 200 µg Ab; 10 µg BP | $361.00 | |||
CBX8 Antibody (C-3) AC | sc-374332 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CBX8 Antibody (C-3) HRP | sc-374332 HRP | 200 µg/ml | $322.00 | |||
CBX8 Antibody (C-3) FITC | sc-374332 FITC | 200 µg/ml | $336.00 | |||
CBX8 Antibody (C-3) PE | sc-374332 PE | 200 µg/ml | $349.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 488 | sc-374332 AF488 | 200 µg/ml | $364.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 546 | sc-374332 AF546 | 200 µg/ml | $364.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 594 | sc-374332 AF594 | 200 µg/ml | $364.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 647 | sc-374332 AF647 | 200 µg/ml | $364.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 680 | sc-374332 AF680 | 200 µg/ml | $364.00 | |||
CBX8 Antibody (C-3) Alexa Fluor® 790 | sc-374332 AF790 | 200 µg/ml | $364.00 | |||
CBX8 (C-3) Neutralizing Peptide | sc-374332 P | 100 µg/0.5 ml | $69.00 |