CBP/KAT3A/CREBBP Double Nickase Plasmid (h): sc-400200-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBP/KAT3A/CREBBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBP/KAT3A/CREBBP Double Nickase Plasmid (h) and CBP/KAT3A/CREBBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CREBBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBP/KAT3A/CREBBP Antibody (C-1): sc-7300
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBP/KAT3A/CREBBP Double Nickase Plasmid (h) | sc-400200-NIC | 20 µg | $410.00 | |||
CBP/KAT3A/CREBBP Double Nickase Plasmid (h2) | sc-400200-NIC-2 | 20 µg | $410.00 |
CREBBP encodes CREB-binding protein (CBP/KAT3A), a nuclear transcriptional coactivator and lysine acetyltransferase that acetylates histones and numerous non-histone substrates to regulate chromatin accessibility and gene expression. CBP integrates signals from CREB, nuclear receptors, and developmental transcription factors, shaping programs controlling cell-cycle progression, differentiation, and DNA damage responses. Through its bromodomain and KAT activity, CBP coordinates enhancer function and transcriptional elongation across multiple signaling networks, including cAMP/CREB-dependent pathways. Altered CREBBP function and epigenetic dysregulation are associated with neurodevelopmental phenotypes and diverse malignancies, making it a widely studied node in transcriptional control and chromatin biology.
CBP/KAT3A/CREBBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CREBBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CREBBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CREBBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CREBBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.