Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
casein kinase IIβ Double Nickase Plasmid (h): sc-401684-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- casein kinase IIβ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- casein kinase IIβ Double Nickase Plasmid (h) and casein kinase IIβ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSNK2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: casein kinase IIβ Antibody (6D5): sc-12739
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase IIβ Double Nickase Plasmid (h) | sc-401684-NIC | 20 µg | $410.00 | |||
casein kinase IIβ Double Nickase Plasmid (h2) | sc-401684-NIC-2 | 20 µg | $410.00 |
CSNK2B encodes the beta regulatory subunit of protein kinase CK2, a constitutively active serine/threonine kinase complex that modulates substrate selection, stability, and assembly of the catalytic subunits. CK2 signaling influences phosphorylation-dependent control of cell cycle progression, DNA damage responses, apoptosis, and RNA processing, interfacing with pathways such as NF-κB, Wnt/β-catenin, and PI3K/AKT. Through broad phosphoregulation of transcription factors and chromatin-associated proteins, CSNK2B contributes to cellular stress adaptation and proteostasis. Dysregulated CK2 activity and altered CSNK2B expression have been linked to oncogenic signaling programs and neurodevelopmental phenotypes, making it a relevant target for mechanistic studies of phosphorylation networks.
casein kinase IIβ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSNK2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSNK2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSNK2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSNK2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.