Cas9 Antibody (7A9-3A3)recommended for detection of transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) of Streptococcus pyogenes origin by WB, IP, IF, IHC(P) and FCM
Cas9 Antibody (7A9-3A3) is a mouse monoclonal IgG1 (kappa light chain) Cas9 antibody provided at 200 µg/ml
raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin
Cas9 Antibody (7A9-3A3) is recommended for detection of transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) of Streptococcus pyogenes origin by WB, IP, IF, IHC(P) and FCM
Cas9 Antibody (7A9-3A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cas9 (7A9-3A3): sc-517386.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Cas9 Antibody (7A9-3A3) is a high quality monoclonal Cas9 antibody suitable for the detection of the Cas9 antigen. Cas9 (CRISPR-associated endonuclease Cas9/Csn1) is a 1,368 amino acid RNA-guided endonuclease of Streptococcus pyogenes origin. Exclusively associated with the type II CRISPR locus, Cas9 nuclease consists of an RNA binding domain, an alpha helix recognition lobe (REC), a nuclease lobe that includes RuvC and HNH for DNA cleavage, and a protospacer adjacent motif (PAM) interacting site. Cas9 nuclease is involved in catalyzing site-specific double strand DNA cleavage. Characterized as being efficient in targeting genomic loci, Cas9 nuclease facilitates targeted genome editing. Cas9 nuclease along with short CRISPR RNAs (crRNAs) function as an RNA-guided endonuclease with RNA-directed target sequence recognition and protein-mediated DNA cleavage in the CRISPR/Cas system. The CRISPR/Cas system is an adaptive immune defense mechanism used by Archea and bacteria for the degradation of foreign genetic material, and has been manipulated to become a powerful tool in genomic engineering.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-11-07
As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.
Asked by: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03
Is there any validation of this antibody working for immunofluorescence applications?
Asked by: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Answered by: Technical Support
Date published: 2019-03-13
What part of the N-terminus was this antibody raised against?
Asked by: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Rated 5 out of
This antibody sample worked wellThis cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459.
1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date published: 2018-09-25
Rated 5 out of
Wubin Ma from
Great antibodyI got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date published: 2018-02-22
Rated 4 out of
Date published: 2017-09-27
Rated 5 out of
Works good.It shows a band at around 160 kDa, and some other bands, probably not specific, because I used high concentration of 1:300
Date published: 2017-08-11
Rated 5 out of
successful resultI have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃，if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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