Cas9 Antibody (7A9-3A3) recommended for detection of transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) of Streptococcus pyogenes origin by WB, IP, IF, IHC(P) and FCM

Cas9 Antibody (7A9-3A3): sc-517386

Cas9 Antibody (7A9-3A3) is rated 4.8 out of 5 by 5.
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Datasheets

    • Cas9 Antibody (7A9-3A3) is a mouse monoclonal IgG1 (kappa light chain) Cas9 antibody provided at 200 µg/ml
    • raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin
    • Cas9 Antibody (7A9-3A3) is recommended for detection of transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) of Streptococcus pyogenes origin by WB, IP, IF, IHC(P) and FCM
    • Cas9 Antibody (7A9-3A3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cas9 (7A9-3A3): sc-517386.
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Do you provide sample for us to try? Thank you!

Asked by: Longjun
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-11-07

As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.

Asked by: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03

Is there any validation of this antibody working for immunofluorescence applications?

Asked by: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Answered by: Technical Support
Date published: 2019-03-13

What part of the N-terminus was this antibody raised against?

Asked by: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Answered by: Technical Support
Date published: 2017-07-26
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Rated 5 out of 5 by from This antibody sample worked well This cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459. 1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date published: 2018-09-25
Rated 5 out of 5 by from Great antibody I got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date published: 2018-02-22
Rated 4 out of 5 by from 还不错 我一个月前申请可改抗体的试用装,很高兴可以提供试用反馈,这个抗体总体不错,有杂带,但是主带清晰,不受干扰。
Date published: 2017-09-27
Rated 5 out of 5 by from Works good. It shows a band at around 160 kDa, and some other bands, probably not specific, because I used high concentration of 1:300
Date published: 2017-08-11
Rated 5 out of 5 by from successful result I have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃,if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
Date published: 2017-07-23
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