Cardiotin Antibody (2Q2272): sc-70494

Cardiotin Antibody (2Q2272) is a mouse monoclonal IgM antibody that detects Cardiotin in mouse, rat, human, porcine, avian, Xenopus, feline, and canine samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Cardiotin is a high molecular weight protein complex located in the mitochondrial membrane, where Cardiotin plays a crucial role in maintaining mitochondrial function and energy production, essential for the proper functioning of cardiac cells. The structure of Cardiotin consists of two subunits that share a highly conserved N-terminal 14 amino acid sequence, demonstrating significant homology to human skeletal muscle α-actinin. This tetrameric configuration forms a transmembrane complex with the N-terminus positioned on the cytoplasmic side of the membrane, allowing Cardiotin to interact effectively with actin filaments. Disruption of Cardiotin distribution in pathological cardiomyocytes, such as those found in chronic ischemic myocardium, can lead to impaired mitochondrial activity and contribute to cardiac contractile dysfunction. Anti-Cardiotin antibody (2Q2272) is particularly valuable in immunohistochemistry with paraffin-embedded sections (IHCP) for identifying alterations in mitochondrial activity in cardiomyocytes during conditions like chronic ischemia or chronic atrial fibrillation, providing insights into the underlying mechanisms of cardiac diseases.

The preferred Cardiotin antibody is Cardiotin Antibody (R2G): sc-53001.