Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKII beta Antibody (D-6): sc-376828. Western blot analysis of CaMKIIβ expression in untreated HeLa (A), chemically-treated HeLa (B, C), untreated K-562 (D) and chemically-treated K-562 (E) whole cell lysates. Detection reagent used: m-IgGκ BP-HRP: sc-516102. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CaMKII beta Double Nickase Plasmid (h): sc-402744-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CaMKII beta Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CaMKII beta Double Nickase Plasmid (h) and CaMKII beta Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAMK2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CaMKII beta Antibody (K-19): sc-100366
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CaMKII beta Double Nickase Plasmid (h) | sc-402744-NIC | 20 µg | $410.00 | |||
CaMKII beta Double Nickase Plasmid (h2) | sc-402744-NIC-2 | 20 µg | $410.00 |