CALM Antibody (G-8): sc-390580
- CALM Antibody (G-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 287-420 mapping within an internal region of CALM of human origin
- recommended for detection of CALM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for CALM Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with CALM Antibody (G-8) (see ordering information below).
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CALM Antibody (G-8) is a mouse monoclonal IgG1 antibody that detects CALM in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CALM, or clathrin assembly protein lymphoid myeloid leukemia, plays a crucial role in the clathrin-mediated endocytosis process, which is essential for cellular uptake of nutrients and signaling molecules. CALM (G-8) antibody recognizes a protein primarily located in the cytoplasm involved in the assembly of clathrin-coated pits, facilitating the formation of vesicles that transport cargo from the plasma membrane and trans-Golgi network. CALM′s cellular location enables interaction with clathrin and other adapter proteins, such as AP180, to ensure efficient vesicle formation and trafficking. Disruptions in this process can lead to various cellular dysfunctions and diseases, highlighting CALM′s significance in maintaining cellular homeostasis. Anti-CALM antibody (G-8) serves as an invaluable tool for researchers studying endocytic pathways and related cellular mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CALM Antibody (G-8) References:
- Evidence for CALM in directing VAMP2 trafficking. | Harel, A., et al. 2008. Traffic. 9: 417-29. PMID: 18182011
- SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses. | Koo, SJ., et al. 2011. Proc Natl Acad Sci U S A. 108: 13540-5. PMID: 21808019
- The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice. | Suzuki, M., et al. 2012. PLoS One. 7: e31854. PMID: 22363754
- FHL2 interacts with CALM and is highly expressed in acute erythroid leukemia. | Pašaliç, Z., et al. 2011. Blood Cancer J. 1: e42. PMID: 22829078
- Abrogation of MLL-AF10 and CALM-AF10-mediated transformation through genetic inactivation or pharmacological inhibition of the H3K79 methyltransferase Dot1l. | Chen, L., et al. 2013. Leukemia. 27: 813-22. PMID: 23138183
- Nuclear export signal within CALM is necessary for CALM-AF10-induced leukemia. | Suzuki, M., et al. 2014. Cancer Sci. 105: 315-23. PMID: 24397609
- The Biochemical Properties and Functions of CALM and AP180 in Clathrin Mediated Endocytosis. | Moshkanbaryans, L., et al. 2014. Membranes (Basel). 4: 388-413. PMID: 25090048
- The ∼ 16 kDa C-terminal sequence of clathrin assembly protein AP180 is essential for efficient clathrin binding. | Chan, LS., et al. 2014. PLoS One. 9: e110557. PMID: 25329427
- Chlorpromazine eliminates acute myeloid leukemia cells by perturbing subcellular localization of FLT3-ITD and KIT-D816V. | Rai, S., et al. 2020. Nat Commun. 11: 4147. PMID: 32811837
- The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family. | Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CALM Antibody (G-8) | sc-390580 | 200 µg/ml | $322.00 | |||
CALM Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-540820 | 200 µg Ab; 10 µg BP | $361.00 |