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Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses. CalciFluor™ Fluo-8 are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength of maximum excitation at ~490 nm and maximum emission at ~520 nm. CalciFluor™ Fluo-8 has a dissociation constant of Kd = 389 nM.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CalciFluor™ Fluo-8, sodium salt, 500 µg | sc-362562A | 500 µg | $254.00 | |||
CalciFluor™ Fluo-8, sodium salt, 1 mg | sc-362562 | 1 mg | $369.00 |