CA VI Double Nickase Plasmid (h): sc-405565-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CA VI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CA VI Double Nickase Plasmid (h) and CA VI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CA6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CA VI Antibody (F-12): sc-166679
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CA VI Double Nickase Plasmid (h) | sc-405565-NIC | 20 µg | $410.00 | |||
CA VI Double Nickase Plasmid (h2) | sc-405565-NIC-2 | 20 µg | $410.00 |
Carbonic anhydrase VI (CA VI), encoded by the human CA6 gene, is a secreted zinc metalloenzyme that catalyzes the reversible hydration of CO₂ to bicarbonate and protons, contributing to extracellular pH homeostasis and buffer capacity in saliva and other glandular secretions. By regulating local acid–base balance, CA VI influences processes linked to mucosal surface chemistry, enamel demineralization/remineralization dynamics, and microenvironmental conditions that shape host–microbiome interactions. Altered CA6 expression and CA VI activity have been associated with variation in oral physiological traits, including salivary buffering and susceptibility to pH-dependent tissue changes, supporting its utility as a molecular readout of secretory gland function and epithelial homeostasis. These features make CA6 a relevant target for mechanistic studies of extracellular carbonic anhydrase biology, pH-regulated signaling, and secreted enzyme contributions to barrier-adjacent microenvironments.
CA VI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CA6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CA6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CA6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CA6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.