Blotto, non-fat dry milk: sc-2324
- Blotto, non-fat dry milk is supplied as a lyophilized powder in a single-use bottle; available in 2 sizes: 250 g and 500 g
- Recommended to prepare both our TBS Blotto A (sc-2333) and TBS Blotto B (sc-2335) blocking solutions
- Blotto, non-fat dry milk is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and background
- Recommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using HRP conjugated primary and secondary antibodies, or mouse IgG binding proteins
- If using direct conjugated primary antibodies or for near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent (sc-516214)
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Blotto, commonly made with non-fat dry milk, is a blocking solution widely used in various immunoassay techniques, including Western blotting and enzyme-linked immunosorbent assays (ELISA). Non-fat dry milk is dissolved in a buffer, often Tris-buffered saline (TBS) or phosphate-buffered saline (PBS), to create Blotto. This solution serves as a blocking agent to prevent non-specific binding of antibodies to the membrane or plate surface during immunoassays. Blotto effectively blocks unoccupied binding sites on the membrane or plate, reducing background noise and increasing the specificity and sensitivity of antibody detection. It is typically added after the primary antibody incubation step and before the secondary antibody incubation step in immunoassay protocols. In addition to non-fat dry milk, other blocking agents such as bovine serum albumin (BSA) or gelatin may also be used to prepare Blotto, depending on the specific requirements of the assay and the target antigen.
Blotto, non-fat dry milk References:
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- Effects of mercury, methylmercury and aluminium on glial fibrillary acidic protein expression in rat cerebellar astrocyte cultures. | Toimela, TA. and Tähti, H. 1995. Toxicol In Vitro. 9: 317-25. PMID: 20650093
- S-nitrosoglutathione reduces oxidative injury and promotes mechanisms of neurorepair following traumatic brain injury in rats. | Khan, M., et al. 2011. J Neuroinflammation. 8: 78. PMID: 21733162
- Osteopontin regulates α-smooth muscle actin and calponin in vascular smooth muscle cells. | Gao, H., et al. 2012. Cell Biol Int. 36: 155-61. PMID: 22032345
- Enzymatic treatment of peanut kernels to reduce allergen levels. | Yu, J., et al. 2011. Food Chem. 127: 1014-22. PMID: 25214091
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- Oxytocin receptor gene loss influences expression of the oxytocin gene in C57BL/6J mice in a sex- and age-dependent manner. | Vaidyanathan, R. and Hammock, EAD. 2020. J Neuroendocrinol. 32: e12821. PMID: 31845417
- Alternative splicing mediates the compensatory upregulation of MBNL2 upon MBNL1 loss-of-function. | Nitschke, L., et al. 2023. Nucleic Acids Res. 51: 1245-1259. PMID: 36617982
- Herpes simplex virus DNA in normal corneas: persistence without viral shedding from ganglia. | Openshaw, H., et al. 1995. J Med Virol. 46: 75-80. PMID: 7623011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Blotto, non-fat dry milk, 250 g | sc-2324 | 250 g | $36.00 | |||
Blotto, non-fat dry milk, 500 g | sc-2325 | 500 g | $65.00 | |||
Blotto, non-fat dry milk, 5 kg | sc-2325A | 5 kg | $562.00 |