Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BLM Double Nickase Plasmid (h): sc-400896-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BLM Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BLM Double Nickase Plasmid (h) and BLM Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BLM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BLM Antibody (B-4): sc-365753
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BLM Double Nickase Plasmid (h) | sc-400896-NIC | 20 µg | $410.00 | |||
BLM Double Nickase Plasmid (h2) | sc-400896-NIC-2 | 20 µg | $410.00 |
BLM encodes a RecQ family DNA helicase that preserves genome stability by unwinding aberrant DNA secondary structures and coordinating DNA end resection during homologous recombination. BLM functions with the BTRR complex (BLM–TOP3A–RMI1/2) to dissolve double Holliday junctions, limiting crossover events and promoting faithful chromosome segregation. It is integrated into S-phase checkpoint control, stalled replication fork restart, and telomere maintenance pathways that constrain chromosomal breakage and sister chromatid exchange. Loss or dysfunction of BLM perturbs replication stress responses and is linked to Bloom syndrome–associated genomic instability phenotypes and increased cancer predisposition in experimental models.
BLM Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BLM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BLM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BLM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BLM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.