Bis(2-cyanoethyl)-N,N-diisopropyl Phosphoramidite (CAS 102690-88-0)

Bis(2-cyanoethyl)-N,N-diisopropyl Phosphoramidite is a reagent widely used in the field of oligonucleotide synthesis, especially in the automated synthesis of DNA and RNA sequences. Its role in the chemical assembly of nucleic acids is critical, as it enables the formation of the phosphate backbone of the oligonucleotide chain through phosphoramidite chemistry. Researchers rely on this compound for its efficiency in producing high-fidelity sequences during solid-phase synthesis protocols. The cyanoethyl groups serve as temporary protecting groups that ensure the selectivity of the reaction and prevent unwanted side reactions. This specificity is essential for constructing long chains of nucleotides with precise sequences. Additionally, this phosphoramidite is involved in the synthesis of modified oligonucleotides, which are instrumental in studying structure-function relationships in nucleic acids, probing molecular interactions, and developing nucleic acid-based assays.