BF-1 Antibody (D-12): sc-518188

BF-1 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects BF-1 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BF-1 antibody (D-12) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BF-1, also known as brain factor 1 or foxg1, is a winged-helix transcriptional repressor that plays a pivotal role in progenitor cell proliferation within the cerebral cortex and is crucial for proper regional patterning of the mammalian telencephalon. BF-1 is characterized by a highly conserved WH domain, essential for DNA-binding capabilities, allowing regulation of gene expression during critical stages of brain development. BF-1 is primarily located in telencephalic progenitor cell nuclei, where BF-1 influences neuron differentiation and maturation. Precise regulation of BF-1 is vital, as disruption can lead to severe developmental defects in the cerebral cortex and basal ganglia, underscoring BF-1′s importance in neurogenesis. BF-1 expression begins as early as embryonic day 8.5, indicating early involvement in brain development, and may modulate progenitor cell responses to environmental signals, highlighting BF-1′s significance in neurodevelopmental processes.

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BF-1 Antibody (D-12) References:

1. Dynamics of placodal lineage development revealed by targeted transgene expression.  |  Hatini, V., et al. 1999. Dev Dyn. 215: 332-43. PMID: 10417822
2. Unified nomenclature for the winged helix/forkhead transcription factors.  |  Kaestner, KH., et al. 2000. Genes Dev. 14: 142-6. PMID: 10702024
3. BF-1 interferes with transforming growth factor beta signaling by associating with Smad partners.  |  Dou, C., et al. 2000. Mol Cell Biol. 20: 6201-11. PMID: 10938097
4. The winged-helix protein brain factor 1 interacts with groucho and hes proteins to repress transcription.  |  Yao, J., et al. 2001. Mol Cell Biol. 21: 1962-72. PMID: 11238932
5. Bifidobacterium bifidum BF-1 suppresses Helicobacter pylori-induced genes in human epithelial cells.  |  Shirasawa, Y., et al. 2010. J Dairy Sci. 93: 4526-34. PMID: 20854986
6. Effect of Bifidobacterium bifidum BF-1 on gastric protection and mucin production in an acute gastric injury rat model.  |  Gomi, A., et al. 2013. J Dairy Sci. 96: 832-7. PMID: 23200466
7. Accuracy of respiratory gas variables, substrate, and energy use from 15 CPET systems during simulated and human exercise.  |  Van Hooren, B., et al. 2024. Scand J Med Sci Sports. 34: e14490. PMID: 37697640
8. Winged helix transcription factor BF-1 is essential for the development of the cerebral hemispheres.  |  Xuan, S., et al. 1995. Neuron. 14: 1141-52. PMID: 7605629
9. Expression of winged helix genes, BF-1 and BF-2, define adjacent domains within the developing forebrain and retina.  |  Hatini, V., et al. 1994. J Neurobiol. 25: 1293-309. PMID: 7815060
10. Regionalization of the developing forebrain: a comparison of FORSE-1, Dlx-2, and BF-1.  |  Tole, S. and Patterson, PH. 1995. J Neurosci. 15: 970-80. PMID: 7869122
11. Longitudinal organization of the anterior neural plate and neural tube.  |  Shimamura, K., et al. 1995. Development. 121: 3923-33. PMID: 8575293
12. Five years on the wings of fork head.  |  Kaufmann, E. and Knöchel, W. 1996. Mech Dev. 57: 3-20. PMID: 8817449

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BF-1 Antibody (D-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-BF-1 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BF-1 Antibody (D-12) Specifications

Alternate Product Name: BF-1

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 268-294

Epitope Target: BF-1

Epitope Species: human

Product Reactivity/Detection: BF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for BF-1 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin