Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta Synuclein Double Nickase Plasmid (h): sc-400894-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta Synuclein Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- beta Synuclein Double Nickase Plasmid (h) and beta Synuclein Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNCB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta Synuclein Antibody (8): sc-136452
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Synuclein Double Nickase Plasmid (h) | sc-400894-NIC | 20 µg | $410.00 | |||
beta Synuclein Double Nickase Plasmid (h2) | sc-400894-NIC-2 | 20 µg | $410.00 |
Human SNCB encodes beta-synuclein, a presynaptic cytosolic protein enriched in neurons where it contributes to synaptic vesicle cycling, membrane-associated protein interactions, and modulation of neurotransmitter release. As a member of the synuclein family, it interfaces with proteostasis networks including chaperone-mediated folding, ubiquitin–proteasome turnover, and autophagy-lysosome pathways that influence protein aggregation dynamics. Beta-synuclein is studied in the context of neuronal vulnerability and stress responses, with relevance to neurodegenerative disease mechanisms involving synaptic dysfunction and misfolded protein handling. Its expression and biochemical interactions provide a framework for dissecting how presynaptic homeostasis impacts neuronal circuitry and cell survival pathways.
beta Synuclein Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNCB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNCB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNCB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNCB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.