β-Galactosidase Antibody (BG-02): sc-51599

β-Galactosidase Antibody (BG-02) is a mouse monoclonal IgG1 antibody that detects β-Galactosidase in E. coli by western blotting (WB) and immunofluorescence (IF). β-Galactosidase, also known as LacZ, plays a crucial role in the hydrolysis of terminal galactose residues, which is essential for various biological processes, including the metabolism of lactose in bacteria. This enzyme operates optimally at a pH range of 6 to 8 and is a tetramer composed of four identical subunits, each containing an active site capable of independently catalyzing the enzymatic reaction. The presence of monovalent cations enhances β-Galactosidase activity, likely through the formation of a galactosyl-enzyme complex intermediate. Due to widespread occurrence in animals, microorganisms, and plants, the LacZ gene has become a valuable tool in molecular biology, particularly as a reporter gene. LacZ is frequently utilized in plasmid vectors, allowing researchers to visualize gene expression through the production of colored or fluorescent compounds, such as the blue color generated from the substrate X-gal. The versatility and importance of β-Galactosidase in various applications underscore the significance of β-Galactosidase (BG-02) antibody in research and diagnostics.

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β-Galactosidase Antibody (BG-02) References:

1. Plasma Escherichia coli beta-galactosidase as a marker of tumor burden and response to experimental anti-neoplastic therapy in nude mice xenografted with lacZ transduced human tumor cells.  |  Holst-Hansen, C., et al. 2000. Lab Invest. 80: 719-24. PMID: 10830782
2. Luminometric quantitation of photinus pyralis firefly luciferase and Escherichia coli beta-galactosidase in blood-contaminated organ lysates.  |  Smith, AD. and Trempe, JP. 2000. Anal Biochem. 286: 164-72. PMID: 11038287
3. Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products.  |  Dráber, P., et al. 1992. Hybridoma. 11: 385-90. PMID: 1500073
4. Protein denaturation during heat shock and related stress. Escherichia coli beta-galactosidase and Photinus pyralis luciferase inactivation in mouse cells.  |  Nguyen, VT., et al. 1989. J Biol Chem. 264: 10487-92. PMID: 2499580
5. Grafting genetically modified cells into the rat brain: characteristics of E. coli beta-galactosidase as a reporter gene.  |  Shimohama, S., et al. 1989. Brain Res Mol Brain Res. 5: 271-8. PMID: 2501628
6. Identification of pre- and pro-haptens with a β-galactosidase-expressing E. coli culture system for skin sensitization.  |  Nepal, MR., et al. 2019. Toxicol Lett. 305: 81-93. PMID: 30711672
7. Dihydrofolate reductase (mouse) and beta-galactosidase (Escherichia coli) can be translocated across the plasma membrane of E. coli.  |  Freudl, R., et al. 1988. J Biol Chem. 263: 17084-91. PMID: 3141414
8. Strong and regulated expression of Escherichia coli beta-galactosidase in insect cells with a baculovirus vector.  |  Pennock, GD., et al. 1984. Mol Cell Biol. 4: 399-406. PMID: 6325875
9. An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label.  |  Comoglio, S. and Celada, F. 1976. J Immunol Methods. 10: 161-70. PMID: 778271
10. Expression of Escherichia coli beta-galactosidase and rat HPRT in the CNS of Macaca mulatta following adenoviral mediated gene transfer.  |  Davidson, BL., et al. 1994. Exp Neurol. 125: 258-67. PMID: 8313940

The preferred β-Galactosidase antibody is β-Galactosidase Antibody (40-1a): sc-65670.