beta-catenin Antibody (A-12): sc-515105

β-catenin Antibody (A-12) is a mouse monoclonal IgG3 kappa light chain antibody engineered to detect β-catenin, also known as CTNNB1, a pivotal protein involved in cellular adhesion and signaling pathways. β-catenin is a key component of the cadherin protein complex at the cell membrane, where β-catenin interacts with E-cadherin to maintain strong cell-cell adhesion and ensure tissue integrity. Additionally, β-catenin plays a central role in the Wnt signaling pathway by interacting with transcription factors such as TCF/LEF to regulate gene expression critical for cell proliferation, differentiation, and migration. β-catenin also binds to the tumor suppressor APC (adenomatous polyposis coli), which is essential for regulating β-catenin levels and preventing uncontrolled cell growth. Furthermore, β-catenin interacts with co-activators like CBP/p300 in the nucleus to modulate transcriptional activity, and with kinases such as GSK-3β, which phosphorylate β-catenin to target β-catenin for ubiquitination and degradation. β-catenin monoclonal antibody (A-12) specifically recognizes an epitope between amino acids 684-707 near the C-terminus of human β-catenin, ensuring precise and reliable detection in multiple species including mouse, rat, and human. Anti-β-catenin antibody (A-12) is validated for a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), making anti-β-catenin antibody (A-12) a versatile tool for diverse research needs. By enabling the detailed study of β-catenin′s interactions with other proteins and role in critical cellular processes, β-catenin (A-12) antibody is an indispensable reagent for researchers investigating the molecular mechanisms underlying cellular communication, development, and oncogenesis.

The preferred beta-catenin antibody is beta-catenin Antibody (E-5): sc-7963.