Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta Actin CRISPR/Cas9 KO Plasmid (m): sc-418965
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta Actin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the beta Actin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta Actin Antibody (C4): sc-47778
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Actin CRISPR/Cas9 KO Plasmid (m) | sc-418965 | 20 µg | $397.00 |
Overview
Actb encodes mouse beta actin, a highly conserved cytoskeletal protein that polymerizes into microfilaments to support cell shape, polarity, and mechanical stability. Beta actin dynamics drive core processes including cytokinesis, vesicle trafficking, and cell migration through coordinated regulation of actin nucleation, branching, and turnover in pathways involving Rho family GTPases and actin-binding proteins. Beyond structural roles, actin remodeling influences transcriptional programs and signal transduction by modulating mechanotransduction and nuclear actin pools. Dysregulated actin organization is broadly implicated in experimental models of developmental defects, impaired wound repair, immune cell motility changes, and tumor cell invasion, making Actb a key reference locus in cell biology and disease mechanism studies.
beta Actin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Actb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Actb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Actb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish beta Actin protein expression.
This CRISPR knockout system enables efficient generation of Actb-deficient cell models for investigation of beta Actin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Actb exon(s) critical for beta Actin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Actb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by beta Actin CRISPR/Cas9 KO Plasmid (m) and beta Actin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Actb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by beta Actin HDR Plasmid (m) and beta Actin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Actb homology arms to support homology-directed repair at defined Actb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.