Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BCDIN3D CRISPR/Cas9 KO Plasmid (h): sc-406497
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BCDIN3D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BCDIN3D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BCDIN3D Antibody (F-5): sc-390348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BCDIN3D CRISPR/Cas9 KO Plasmid (h) | sc-406497 | 20 µg | $397.00 |
Overview
BCDIN3D (BCDIN3D RNA methyltransferase) is a SAM-dependent methyltransferase implicated in RNA end modification, with reported activity toward the 5′-monophosphate of specific precursor microRNAs. By altering miRNA maturation and small RNA stability, BCDIN3D can influence post-transcriptional gene regulation programs that shape cell-state transitions, proliferation, and stress responses. Functional studies have linked dysregulated BCDIN3D expression to altered miRNA profiles and phenotypes relevant to tumor biology, including changes in invasion and metastatic potential in select cellular contexts. As a result, BCDIN3D is frequently examined in pathways connecting RNA processing to oncogenic signaling and epigenetic-like control of gene expression.
BCDIN3D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BCDIN3D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BCDIN3D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BCDIN3D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BCDIN3D protein expression.
This CRISPR knockout system enables efficient generation of BCDIN3D-deficient cell models for investigation of BCDIN3D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BCDIN3D exon(s) critical for BCDIN3D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BCDIN3D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BCDIN3D CRISPR/Cas9 KO Plasmid (h) and BCDIN3D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BCDIN3D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BCDIN3D HDR Plasmid (h) and BCDIN3D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BCDIN3D homology arms to support homology-directed repair at defined BCDIN3D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.