Barx1 CRISPR/Cas9 KO Plasmid (m): sc-419290
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Barx1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Barx1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Barx1 Antibody (392.8): sc-81956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Barx1 CRISPR/Cas9 KO Plasmid (m) | sc-419290 | 20 µg | $397.00 |
Overview
Barx1 (BARX homeobox 1) is a homeobox transcription factor that helps regulate embryonic patterning and organogenesis in mouse, with prominent roles in craniofacial development and foregut-derived tissues. By binding DNA through its homeodomain, Barx1 influences transcriptional programs that coordinate epithelial–mesenchymal interactions, cell fate specification, and extracellular matrix remodeling during morphogenesis. Barx1 activity intersects with developmental signaling networks such as Wnt/β-catenin and BMP pathways, where it can shape differentiation outcomes and tissue boundary formation. Dysregulation of Barx1-associated gene networks is relevant to studies of congenital malformations and developmental disorders affecting facial and gastrointestinal structures.
Barx1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Barx1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Barx1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Barx1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Barx1 protein expression.
This CRISPR knockout system enables efficient generation of Barx1-deficient cell models for investigation of Barx1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Barx1 exon(s) critical for Barx1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Barx1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Barx1 CRISPR/Cas9 KO Plasmid (m) and Barx1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Barx1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Barx1 HDR Plasmid (m) and Barx1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Barx1 homology arms to support homology-directed repair at defined Barx1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.