BAG-6 Antibody (D-1): sc-365928

BAG-6 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects BAG-6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). BAG-6 (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BAG-6 protein, also known as BAT3 (HLA-B associated transcript 3), plays a crucial role in regulating apoptosis, which is vital for maintaining cellular homeostasis and preventing tumorigenesis. Located primarily in the nucleus, BAG-6 contains a ubiquitin-like domain at its N-terminus and two nuclear localization signals at its C-terminus, facilitating BAG-6′s function in cellular stress responses. Notably, BAG-6 interacts with apoptosis-inducing factor (AIF), stabilizing AIF and enhancing cellular sensitivity to apoptosis triggered by endoplasmic reticulum stress. In the context of cellular stress, such as during ricin treatment, BAG-6 is cleaved by caspase-3, leading to the generation of a pro-apoptotic fragment that promotes critical apoptotic processes, including nuclear condensation and phosphatidylserine externalization. Mice lacking BAG-6 exhibit severe developmental defects in vital organs such as the lungs, brain, and kidneys, underscoring BAG-6′s essential role in regulating both proliferation and apoptosis, with deficiencies often resulting in perinatal or midgestational lethality.

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BAG-6 Antibody (D-1) References:

1. Cloning and characterization of rat BAT3 cDNA.  |  Ozaki, T., et al. 1999. DNA Cell Biol. 18: 503-12. PMID: 10390159
2. Human Scythe contains a functional nuclear localization sequence and remains in the nucleus during staurosporine-induced apoptosis.  |  Manchen, ST. and Hubberstey, AV. 2001. Biochem Biophys Res Commun. 287: 1075-82. PMID: 11587531
3. Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.  |  Lehner, B., et al. 2004. Genomics. 83: 153-67. PMID: 14667819
4. Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3.  |  Wu, YH., et al. 2004. J Biol Chem. 279: 19264-75. PMID: 14960581
5. Human SGT interacts with Bag-6/Bat-3/Scythe and cells with reduced levels of either protein display persistence of few misaligned chromosomes and mitotic arrest.  |  Winnefeld, M., et al. 2006. Exp Cell Res. 312: 2500-14. PMID: 16777091
6. Phenotype-based identification of host genes required for replication of African swine fever virus.  |  Chang, AC., et al. 2006. J Virol. 80: 8705-17. PMID: 16912318
7. HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53.  |  Sasaki, T., et al. 2007. Genes Dev. 21: 848-61. PMID: 17403783
8. Human leukocyte antigen-B-associated transcript 3 is released from tumor cells and engages the NKp30 receptor on natural killer cells.  |  Pogge von Strandmann, E., et al. 2007. Immunity. 27: 965-74. PMID: 18055229
9. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis.  |  Desmots, F., et al. 2008. J Biol Chem. 283: 3264-3271. PMID: 18056262
10. BAG-6 is essential for selective elimination of defective proteasomal substrates.  |  Minami, R., et al. 2010. J Cell Biol. 190: 637-50. PMID: 20713601
11. A soluble fragment of the tumor antigen BCL2-associated athanogene 6 (BAG-6) is essential and sufficient for inhibition of NKp30 receptor-dependent cytotoxicity of natural killer cells.  |  Binici, J., et al. 2013. J Biol Chem. 288: 34295-303. PMID: 24133212
12. A new cluster of genes within the human major histocompatibility complex.  |  Spies, T., et al. 1989. Science. 243: 214-7. PMID: 2911734

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BAG-6 Antibody (D-1) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-BAG-6 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BAG-6 Antibody (D-1) Specifications

Alternate Product Name: BAG-6

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 833-982

Epitope Target: BAG-6

Epitope Species: human

Product Reactivity/Detection: BAG-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for BAG-6 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin