BAF53 Antibody (B-1): sc-137063
- BAF53 Antibody (B-1) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 87-129 near the N-terminus of BAF53 of human origin
- recommended for detection of BAF53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
- TransCruz reagent for ChIP application (sc-137063 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for BAF53 Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with BAF53 Antibody (B-1) (see ordering information below).
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BAF53 Antibody (B-1) is a mouse monoclonal IgG2a antibody that detects BAF53 protein of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). BAF53, a crucial component of the SWI/SNF chromatin remodeling complex, plays a significant role in regulating gene expression by facilitating ATP-dependent chromatin remodeling, which is essential for proper cellular function and development. BAF53 protein is primarily located in both the nucleus and cytoplasm, allowing BAF53 to shuttle between these compartments and participate in diverse cellular processes. This dynamic localization enables BAF53 protein to interact with various transcription factors and chromatin remodeling complexes, thereby influencing gene expression patterns in response to cellular signals. Notably, BAF53 protein forms a complex with TIP49 and TIP48, which is implicated in mediating the oncogenic activity of c-Myc, highlighting BAF53′s importance in cancer biology. The conserved N-terminal motif of BAF53, which includes critical residues at serine 2 and tyrosine 6, is essential for BAF53′s activity, further underscoring the intricate regulatory mechanisms that govern gene expression and cellular homeostasis.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
BAF53 Antibody (B-1) References:
- Assignment of the SMARCF1 gene to tammar wallaby chromosome 5q by fluorescence in situ hybridisation. | Waters, PD., et al. 2001. Cytogenet Cell Genet. 93: 315-6. PMID: 11528131
- BAF53 forms distinct nuclear complexes and functions as a critical c-Myc-interacting nuclear cofactor for oncogenic transformation. | Park, J., et al. 2002. Mol Cell Biol. 22: 1307-16. PMID: 11839798
- TIP49 regulates beta-catenin-mediated neoplastic transformation and T-cell factor target gene induction via effects on chromatin remodeling. | Feng, Y., et al. 2003. Cancer Res. 63: 8726-34. PMID: 14695187
- Skeletrophin, a novel RING molecule controlled by the chromatin remodeling complex, is downregulated in malignant melanoma. | Takeuchi, T., et al. 2005. DNA Cell Biol. 24: 339-44. PMID: 15869411
- Effects of Ser2 and Tyr6 mutants of BAF53 on cell growth and p53-dependent transcription. | Lee, JH., et al. 2005. Mol Cells. 19: 289-93. PMID: 15879716
- BAF53 interacts with p53 and functions in p53-mediated p21-gene transcription. | Wang, M., et al. 2007. J Biochem. 142: 613-20. PMID: 17878219
- Association of BAF53 with mitotic chromosomes. | Lee, K., et al. 2007. Mol Cells. 24: 288-93. PMID: 17978584
- Recruitment of the SWI/SNF chromatin remodeling complex to steroid hormone-regulated promoters by nuclear receptor coactivator flightless-I. | Jeong, KW., et al. 2009. J Biol Chem. 284: 29298-309. PMID: 19720835
- Suppression of HPV E6 and E7 expression by BAF53 depletion in cervical cancer cells. | Lee, K., et al. 2011. Biochem Biophys Res Commun. 412: 328-33. PMID: 21821000
- DNA Damage Triggers the Nuclear Accumulation of RASSF6 Tumor Suppressor Protein via CDK9 and BAF53 To Regulate p53 Target Gene Transcription. | Kuleape, JA., et al. 2022. Mol Cell Biol. 42: e0031021. PMID: 34898277
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAF53 Antibody (B-1) | sc-137063 | 200 µg/ml | $322.00 | |||
BAF53 Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-539926 | 200 µg Ab; 10 µg BP | $361.00 | |||
BAF53 Antibody (B-1): m-IgG2a BP-HRP Bundle | sc-546820 | 200 µg Ab; 10 µg BP | $361.00 | |||
BAF53 (B-1) Neutralizing Peptide | sc-137063 P | 100 µg/0.5 ml | $69.00 | |||
BAF53 Antibody (B-1) X | sc-137063 X | 200 µg/0.1 ml | $322.00 |