Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AWP1 CRISPR/Cas9 KO Plasmid (m): sc-425748
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AWP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AWP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AWP1 Antibody (B-5): sc-398513
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AWP1 CRISPR/Cas9 KO Plasmid (m) | sc-425748 | 20 µg | $397.00 |
Overview
Zfand6 encodes AWP1, a zinc finger AN1-type domain–containing protein implicated in ubiquitin-dependent regulation of protein turnover and cellular stress signaling. AWP1 has been linked to modulation of inflammatory and innate immune pathways, including NF-κB signaling, through interactions with ubiquitinated substrates and components of ubiquitin ligase networks. In mouse systems, Zfand6 expression and function are studied in the context of proteostasis, signal transduction, and cellular responses to cytokines and stress. Dysregulation of ubiquitin-mediated control and inflammatory signaling is broadly relevant to mechanisms underlying cancer biology, neurodegeneration, and immune-driven pathology, making Zfand6 a useful node for pathway interrogation.
AWP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zfand6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zfand6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zfand6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AWP1 protein expression.
This CRISPR knockout system enables efficient generation of Zfand6-deficient cell models for investigation of AWP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zfand6 exon(s) critical for AWP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zfand6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AWP1 CRISPR/Cas9 KO Plasmid (m) and AWP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zfand6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AWP1 HDR Plasmid (m) and AWP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zfand6 homology arms to support homology-directed repair at defined Zfand6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.