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Aut7 Antibody (B-11): sc-374016

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Datasheets
  • Aut7 Antibody (B-11) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 9-37 near the N-terminus of Aut7 of Saccharomyces cerevisiae origin
  • recommended for detection of Aut7 of y origin by WB, IP, IF and ELISA
  • m-IgG3 BP-HRP is the preferred secondary detection reagent for Aut7 Antibody (B-11) for WB applications. This reagent is now offered in a bundle with Aut7 Antibody (B-11) (see ordering information below).
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    Aut7 Antibody (B-11) is a mouse monoclonal IgG3 antibody that detects Aut7 in yeast by applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Aut7, also known as Atg8, plays a crucial role in the autophagy process, which is essential for cellular homeostasis and the degradation of damaged organelles and proteins. Aut7 is primarily located in the cytoplasm and is involved in the formation of autophagosomes, which are critical for the encapsulation and transport of cellular debris to lysosomes for degradation. Anti-Aut7 antibody (B-11) enables researchers to study proper functioning of Aut7, ensuring that cells can efficiently recycle components and respond to stress conditions, such as nutrient deprivation. Additionally, Aut7 undergoes post-translational modifications, including lipidation, which is necessary for membrane association and function in autophagosome formation. Aut7 monoclonal antibody (B-11) helps researchers gain insights into the mechanisms of autophagy and its implications in various diseases, including neurodegeneration and cancer.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Aut7 Antibody (B-11)

    sc-374016
    200 µg/ml
    $322.00

    Aut7 Antibody (B-11): m-IgG3 BP-HRP Bundle

    sc-550445
    200 µg Ab; 40 µg BP
    $361.00

    Aut7 (B-11) Neutralizing Peptide

    sc-374016 P
    100 µg/0.5 ml
    $69.00

    What application is the blocking peptide sc-374016 P appropriate for?

    Asked by: jenniferc
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28
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    • QUESTIONS, PRODUCT
    Rated 5 out of 5 by from Satisfactory Western Blot data of yeast recombinantSatisfactory Western Blot data of yeast recombinant Aut7 fusion protein. -SCBT QC
    Date published: 2015-05-07
    Rated 5 out of 5 by from Good Western Blot data of Aut7 expressionGood Western Blot data of Aut7 expression in yeast whole cell lysate. -SCBT QC
    Date published: 2013-02-12
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    Aut7 Antibody (B-11) is rated 5.0 out of 5 by 2.
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