ATXN2L Double Nickase Plasmid (h): sc-406313-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATXN2L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATXN2L Double Nickase Plasmid (h) and ATXN2L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATXN2L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATXN2L Double Nickase Plasmid (h) | sc-406313-NIC | 20 µg | $410.00 | |||
ATXN2L Double Nickase Plasmid (h2) | sc-406313-NIC-2 | 20 µg | $410.00 |
ATXN2L (ataxin-2-like) encodes an RNA-binding protein that localizes to cytoplasmic RNA granules and contributes to post-transcriptional control of gene expression, including mRNA stability and translational regulation. It is implicated in stress granule assembly and cellular stress responses, linking ATXN2L to pathways governing RNA metabolism, proteostasis, and adaptation to environmental cues. Through these functions, ATXN2L can influence cell growth and survival programs and is studied in contexts where dysregulated RNA handling and stress granule dynamics contribute to disease-relevant phenotypes. ATXN2L is therefore a useful target for mechanistic studies of ribonucleoprotein complex regulation and signaling cross-talk that shapes cell state.
ATXN2L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATXN2L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATXN2L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATXN2L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATXN2L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.