ATP5G1 Double Nickase Plasmid (h): sc-416816-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ATP5G1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ATP5G1 Double Nickase Plasmid (h) and ATP5G1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP5G1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ATP5G1 Antibody (1A12): sc-293324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ATP5G1 Double Nickase Plasmid (h) | sc-416816-NIC | 20 µg | $410.00 | |||
ATP5G1 Double Nickase Plasmid (h2) | sc-416816-NIC-2 | 20 µg | $410.00 |
ATP5G1 encodes a mitochondrially localized subunit of the F0 sector of ATP synthase (complex V) that contributes to proton translocation and efficient ATP production through oxidative phosphorylation. By supporting coupling of the electron transport chain to ATP synthesis, ATP5G1 influences mitochondrial membrane potential, cellular energy homeostasis, and downstream processes such as reactive oxygen species management and apoptotic susceptibility. Perturbation of ATP synthase function and broader OXPHOS pathways is implicated in mitochondrial dysfunction phenotypes relevant to neurodegeneration, cardiometabolic stress, and cancer cell bioenergetic remodeling. As a core component of mitochondrial energy conversion, ATP5G1 is frequently studied in contexts of metabolic reprogramming, hypoxia adaptation, and mitochondrial quality control.
ATP5G1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP5G1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP5G1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP5G1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP5G1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.