Atg4b Antibody (231CT21.1.7): sc-517310

Atg4b Antibody (231CT21.1.7) is a mouse monoclonal IgM antibody that detects Atg4b protein of human origin by western blotting (WB). Anti-Atg4b antibody (231CT21.1.7) is available as a non-conjugated format. Atg4b protein, also known as ATG4 autophagy related 4 homolog B, APG4B, or AUTL1, plays a crucial role in autophagy process, which is essential for cellular homeostasis and degradation of damaged organelles and proteins. This 393 amino acid protein is primarily located in cytoplasm and belongs to peptidase C54 family, functioning as a cysteine protease. Anti-Atg4b antibody (231CT21.1.7) recognizes a protein responsible for cleaving C-terminal part of target proteins, such as GABARAP and MAP1LC3, which are vital for formation and maturation of autophagosomes. Proper functioning of Atg4b is critical, as Atg4b regulates autophagic process, thereby influencing cellular responses to stress and maintaining cellular integrity. Additionally, Atg4b exists in multiple alternatively spliced isoforms, and Atg4b activity can be inhibited by N-ethylmaleimide, highlighting importance of post-translational modifications in regulatory mechanisms.

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Atg4b Antibody (231CT21.1.7) References:

1. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy.  |  Mariño, G., et al. 2003. J Biol Chem. 278: 3671-8. PMID: 12446702
2. A single protease, Apg4B, is specific for the autophagy-related ubiquitin-like proteins GATE-16, MAP1-LC3, GABARAP, and Apg8L.  |  Hemelaar, J., et al. 2003. J Biol Chem. 278: 51841-50. PMID: 14530254
3. LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation.  |  Kabeya, Y., et al. 2004. J Cell Sci. 117: 2805-12. PMID: 15169837
4. HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.  |  Tanida, I., et al. 2004. J Biol Chem. 279: 36268-76. PMID: 15187094
5. Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes.  |  Tanida, I., et al. 2004. J Biol Chem. 279: 47704-10. PMID: 15355958
6. Structural basis for the specificity and catalysis of human Atg4B responsible for mammalian autophagy.  |  Sugawara, K., et al. 2005. J Biol Chem. 280: 40058-65. PMID: 16183633
7. The crystal structure of human Atg4b, a processing and de-conjugating enzyme for autophagosome-forming modifiers.  |  Kumanomidou, T., et al. 2006. J Mol Biol. 355: 612-8. PMID: 16325851
8. Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3.  |  Tanida, I., et al. 2006. FEBS J. 273: 2553-62. PMID: 16704426