ASA Antibody (H-5): sc-365176
- ASA Antibody (H-5) is a mouse monoclonal IgG1 κ ASA antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 145-273 mapping within an internal region of ASA of human origin
- ASA Antibody (H-5) is recommended for detection of ASA of human origin by WB, IP, IF and ELISA
- Anti-ASA Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ASA Antibody (H-5) for WB applications. These reagents are now offered in bundles with ASA Antibody (H-5) (see ordering information below).
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ASA Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ASA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ASA (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ASA protein, also known as arylsulfatase A, cerebroside-sulfatase, ARSA, or MLD, is a crucial 507 amino acid lysosomal enzyme that plays a significant role in the hydrolysis of cerebroside 3-sulfate into cerebroside and sulfate, utilizing magnesium as a cofactor. This enzymatic activity is vital for the proper metabolism of sulfatides, which are essential components of myelin, the protective sheath surrounding nerve fibers. Impairments in ASA function lead to severe neurological disorders, including metachromatic leukodystrophy (MLD), characterized by progressive ataxia, dementia, seizures, and spastic tetraparesis, ultimately resulting in life-threatening complications. Furthermore, deficiencies in ASA activity are linked to multiple sulfatase deficiency (MSD), a condition that affects the activity of all known sulfatases and manifests with a range of symptoms, including developmental delays and various metabolic disorders. Anti-ASA antibody (H-5) provides researchers with a powerful tool for studying these critical pathways and understanding the underlying mechanisms of related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ASA Antibody (H-5) References:
- Carbohydrate affinity chromatography indicates that arylsulfatase-A from capacitated boar sperm has mannose and N-acetylglucosamine/sialic acid residues. | Jiménez, I., et al. 2006. Arch Androl. 52: 455-62. PMID: 17050327
- Steroid sulfatase, arylsulfatases A and B, galactose-6-sulfatase, and iduronate sulfatase in mammary cells and effects of sulfated and non-sulfated estrogens on sulfatase activity. | Bhattacharyya, S. and Tobacman, JK. 2007. J Steroid Biochem Mol Biol. 103: 20-34. PMID: 17064891
- Ethanol decreases rat hepatic arylsulfatase A activity levels. | Jean, S., et al. 2006. Alcohol Clin Exp Res. 30: 1950-5. PMID: 17067361
- Gene therapy of metachromatic leukodystrophy reverses neurological damage and deficits in mice. | Biffi, A., et al. 2006. J Clin Invest. 116: 3070-82. PMID: 17080200
- Partial cure of established disease in an animal model of metachromatic leukodystrophy after intracerebral adeno-associated virus-mediated gene transfer. | Sevin, C., et al. 2007. Gene Ther. 14: 405-14. PMID: 17093507
- AAV1 mediated co-expression of formylglycine-generating enzyme and arylsulfatase a efficiently corrects sulfatide storage in a mouse model of metachromatic leukodystrophy. | Kurai, T., et al. 2007. Mol Ther. 15: 38-43. PMID: 17164773
- A spontaneously immortalized Schwann cell line to study the molecular aspects of metachromatic leukodystrophy. | Saravanan, K., et al. 2007. J Neurosci Methods. 161: 223-33. PMID: 17204333
- Metabolic correction in oligodendrocytes derived from metachromatic leukodystrophy mouse model by using encapsulated recombinant myoblasts. | Consiglio, A., et al. 2007. J Neurol Sci. 255: 7-16. PMID: 17341424
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ASA Antibody (H-5) | sc-365176 | 200 µg/ml | $316.00 | |||
ASA Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522152 | 200 µg Ab, 40 µg BP | $354.00 | |||
ASA Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-533520 | 200 µg Ab; 20 µg BP | $354.00 | |||
ASA Antibody (H-5) AC | sc-365176 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ASA Antibody (H-5) HRP | sc-365176 HRP | 200 µg/ml | $316.00 | |||
ASA Antibody (H-5) FITC | sc-365176 FITC | 200 µg/ml | $330.00 | |||
ASA Antibody (H-5) PE | sc-365176 PE | 200 µg/ml | $343.00 | |||
ASA Antibody (H-5) Alexa Fluor® 488 | sc-365176 AF488 | 200 µg/ml | $357.00 | |||
ASA Antibody (H-5) Alexa Fluor® 546 | sc-365176 AF546 | 200 µg/ml | $357.00 | |||
ASA Antibody (H-5) Alexa Fluor® 594 | sc-365176 AF594 | 200 µg/ml | $357.00 | |||
ASA Antibody (H-5) Alexa Fluor® 647 | sc-365176 AF647 | 200 µg/ml | $357.00 | |||
ASA Antibody (H-5) Alexa Fluor® 680 | sc-365176 AF680 | 200 µg/ml | $357.00 | |||
ASA Antibody (H-5) Alexa Fluor® 790 | sc-365176 AF790 | 200 µg/ml | $357.00 |