Aquaporin 2/AQP2 Double Nickase Plasmid (h): sc-400402-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 2/AQP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 2/AQP2 Double Nickase Plasmid (h) and Aquaporin 2/AQP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AQP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 2/AQP2 Antibody (E13-21): sc-47710
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 2/AQP2 Double Nickase Plasmid (h) | sc-400402-NIC | 20 µg | $410.00 | |||
Aquaporin 2/AQP2 Double Nickase Plasmid (h2) | sc-400402-NIC-2 | 20 µg | $410.00 |
AQP2 encodes aquaporin‑2, a vasopressin-regulated water channel that mediates transcellular water reabsorption in renal collecting duct principal cells. Its trafficking to the apical membrane is controlled by AVPR2-driven cAMP/PKA signaling and phosphorylation-dependent insertion into and retrieval from vesicles, linking AQP2 to epithelial polarity and osmotic homeostasis. Altered AQP2 expression, localization, or channel function is associated with impaired urinary concentrating ability and contributes to disorders of water balance such as nephrogenic diabetes insipidus and hyponatremic states. As a kidney-enriched membrane protein, AQP2 is also used to interrogate endosomal recycling, membrane protein quality control, and hormone-regulated transport pathways.
Aquaporin 2/AQP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AQP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AQP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AQP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AQP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.