APC11 Antibody (1B4-1A4): sc-517142

APC11 Antibody (1B4-1A4) is a mouse monoclonal IgG1 kappa light chain antibody that detects APC11 of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-APC11 antibody (1B4-1A4) is available in a non-conjugated form, providing researchers with a versatile tool for studying the anaphase-promoting complex (APC), which is crucial for the regulation of the cell cycle. The APC, comprising more than ten subunits, plays a vital role in promoting the separation of sister chromatids during the transition from metaphase to anaphase in mitosis. This process is essential for accurate chromosome segregation, preventing aneuploidy, which can lead to cancer and other diseases. APC11, a RING-H2 finger protein, is integral to the ubiquitination process, facilitating the synthesis of multiubiquitin chains in conjunction with Ubiquitin carrier protein 4 (Ubc4) and ubiquitin-conjugating enzyme (E2). APC activity is tightly regulated by various factors, including CDC20, CDH1, and MAD2, which modulate function to ensure proper cell cycle progression. Specifically, CDC20 and CDH1 activate APC′s cyclin-ubiquitination activity, while MAD2 inhibits by forming a ternary complex with CDC20 and APC, thus preventing premature activation. Understanding the dynamics of APC11 and its interactions is crucial for elucidating the mechanisms of cell cycle regulation and the potential implications in cancer biology.

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APC11 Antibody (1B4-1A4) References:

1. Subunits and substrates of the anaphase-promoting complex.  |  Peters, JM. 1999. Exp Cell Res. 248: 339-49. PMID: 10222126
2. Control of mitotic transitions by the anaphase-promoting complex.  |  Fang, G., et al. 1999. Philos Trans R Soc Lond B Biol Sci. 354: 1583-90. PMID: 10582244
3. The anaphase-promoting complex: new subunits and regulators.  |  Page, AM. and Hieter, P. 1999. Annu Rev Biochem. 68: 583-609. PMID: 10872461
4. The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex.  |  Gmachl, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 8973-8. PMID: 10922056
5. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex.  |  Tang, Z., et al. 2001. Mol Biol Cell. 12: 3839-51. PMID: 11739784
6. The cyclin-ubiquitin ligase activity of cyclosome/APC is jointly activated by protein kinases Cdk1-cyclin B and Plk.  |  Golan, A., et al. 2002. J Biol Chem. 277: 15552-7. PMID: 11859075
7. Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2.  |  Bolte, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 4385-90. PMID: 11917129
8. A subunit of the anaphase-promoting complex is a centromere-associated protein in mammalian cells.  |  Jörgensen, PM., et al. 1998. Mol Cell Biol. 18: 468-76. PMID: 9418894

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-APC11 Antibody (1B4-1A4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-APC11 Antibody (1B4-1A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

APC11 Antibody (1B4-1A4) Specifications

Alternate Product Name: APC11

Product Clone Name: 1B4-1A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-54

Epitope Target: APC11

Epitope Species: human

Product Reactivity/Detection: APC11

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for APC11 Antibody (1B4-1A4): N/A