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Anti-HA Magnetic Beads

Name: Anti-HA Magnetic Beads
Brand: SCBT
SKU: sc-500773
Price: 454.0 USD
Availability: InStock

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Application:

Anti-HA Magnetic Beads is for immunoprecipitation of recombinant HA-tagged proteins

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

* Refer to Certificate of Analysis for lot specific data.

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Description

Technical Information

Safety Information

SDS & Certificate of Analysis

Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators. Features of Anti-HA Magnetic Beads:Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation. Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour. Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments. Versatile—beads are compatible with manual and automated workflows. The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms. Product Details:Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL of HA peptide can be used.

Anti-HA Magnetic Beads References

A role for the small GTPase Rab6 in assembly of human cytomegalovirus. | Indran, SV. and Britt, WJ. 2011. J Virol. 85: 5213-9. PMID: 21411515
Mechanoactivation of the angiotensin II type 1 receptor induces β-arrestin-biased signaling through Gαi coupling. | Wang, J., et al. 2018. J Cell Biochem. 119: 3586-3597. PMID: 29231251
Immunological Comparison of Native and Recombinant Hen's Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis. | De Silva, C., et al. 2018. Nutrients. 10: PMID: 29895783
Thioredoxin interacting protein (Txnip) forms redox sensitive high molecular weight nucleoprotein complexes. | Hirata, CL., et al. 2019. Arch Biochem Biophys. 677: 108159. PMID: 31669268
Protein complexes including PGRMC1 and actin-associated proteins are disrupted by AG-205. | Teakel, SL., et al. 2020. Biochem Biophys Res Commun. 524: 64-69. PMID: 31980178
Crystal structure of the SALL4-pomalidomide-cereblon-DDB1 complex. | Matyskiela, ME., et al. 2020. Nat Struct Mol Biol. 27: 319-322. PMID: 32251415
De novo variants in CUL3 are associated with global developmental delays with or without infantile spasms. | Nakashima, M., et al. 2020. J Hum Genet. 65: 727-734. PMID: 32341456
Photocaged Cell-Permeable Ubiquitin Probe for Temporal Profiling of Deubiquitinating Enzymes. | Gui, W., et al. 2020. J Am Chem Soc. 142: 19493-19501. PMID: 33141564
Disruption of interferon-β production by the Npro of atypical porcine pestivirus. | Mou, C., et al. 2021. Virulence. 12: 654-665. PMID: 33538238
Immunopurification of Mitochondria from Arabidopsis. | Luo, L., et al. 2021. Curr Protoc. 1: e34. PMID: 33555656
A small Ustilago maydis effector acts as a novel adhesin for hyphal aggregation in plant tumors. | Fukada, F., et al. 2021. New Phytol. 231: 416-431. PMID: 33843063
O-GlcNAc modification regulates MTA1 transcriptional activity during breast cancer cell genotoxic adaptation. | Xie, X., et al. 2021. Biochim Biophys Acta Gen Subj. 1865: 129930. PMID: 34019948
The APE2 Exonuclease Is a Client of the Hsp70-Hsp90 Axis in Yeast and Mammalian Cells. | Omkar, S., et al. 2022. Biomolecules. 12: PMID: 35883419
Suppression of vacuolar-type ATPase and induction of endoplasmic reticulum stress by proton pump inhibitors. | Lee, WP. 2022. J Chin Med Assoc. 85: 915-921. PMID: 36150104
A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screening. | Kaufmann, J., et al. 2022. Commun Biol. 5: 1206. PMID: 36352263

Ordering Information

Product Name	Catalog #	UNIT	Price	Qty	FAVORITES
Anti-HA Magnetic Beads, 1 ml	sc-500773	1 ml	$454.00
Anti-HA Magnetic Beads, 5 ml	sc-500773A	5 ml	$1502.00

If my lysate contains 1MMDTT, will it affect the experimental results? If so, is there any way to deal with it，I don't understand the meaning of "just one more step" in your email

Asked by: joy97

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2021-07-23

If my lysate contains 1 mM DTT, will it affect the experimental results? If so, is there any way to deal with it.

Asked by: joy97

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2021-07-23

Hi, I was wondering if you could share with me the details of pre-blocking the beads: -What is the reagent used for pre-blocking? -If BSA is used, how is it immobilized on the surface of the bead?

Asked by: The Scientist

Thank you for your question. During manufacture, the bead is blocked by the covalent attachment of a synthetic polymer. No BSA or other protein is used for blocking.

Answered by: Technical Support

Date published: 2020-02-14

Hello, I was wondering if you could tell me how the anti-HA antibody is immobilized on the beads? Is it a direct covalent bond or is there an "intermediate" antibody (e.g. Sheep anti-mouse IgG) through which the anti-HA is immobilized on the beads?

Asked by: The Scientist

Thank you for your question. They are covalently bound via the primary amine.

Answered by: Tech Service

Date published: 2024-03-08

I wanted to check interaction between HA tagged protein and TAP fusion protein by fishing the HA tagged protein using HA- magnetic beads but I am worried that the protein A of the TAP fusion binds non specifically to the beads

Asked by: Mano123

Thank you for your question. Please contact our Technical Service Department for assistance with this product.

Answered by: Technical Support

Date published: 2019-09-11

1.What's the expiration date of the beads? 2.What' the binding capacity of the beads？ Thank you

Asked by: cyl918

Thank you for your questions. We do not provide an expiration date, however this product is warrantied for one year from the date of purchase. Binding capacity: ≥ 10μg GST-ERK-HA (70kDa fusion protein)/mg of beads

Answered by: Technical Support

Date published: 2019-06-15

What is the binding capacity of the beads?

Asked by: Ceci

Thanks for your question. The binding capacity of the beads is ≥ 10μg HA (70kDa fusion protein)/mg of beads. Please contact our technical service for further information.

Answered by: SCBT Heidelberg Support

Date published: 2019-01-06

What is the bead concentration?

Asked by: chemsd02

Thank you for your question. The concentration of sc-500773 (Anti-HA Magnetic Beads) is 10 mg/mL.

Answered by: Chemical Support 10

Date published: 2017-03-08

Rated 2 out of 5 by Vitorb2 from Mag-beadsThe binding capacity was pretty good, however, the sample beads bound is extremely hard to release. I suggest a new protocol increase the beads release.

Date published: 2017-07-01

Anti-HA Magnetic Beads is rated 2.0 out of 5 by 1.

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Test	Specification	Results
Appearance	Dark brown slurry solution	Complies
Functionality	Magnetic beads specifically immmuoprecipitates HA-tagged protein from an E. coli lysate.	Passes test
Content	1% ± 0.1% solid	0.9%

Anti-HA Magnetic Beads

Anti-HA Magnetic Beads References

SAMPLE Certificate of Analysis (COA)

TEST CONDITIONS

How to request a Certificate of Analysis (COA) when it is not available online?

Anti-HA Magnetic Beads, 1 ml

Anti-HA Magnetic Beads, 5 ml

If my lysate contains 1MMDTT, will it affect the experimental results? If so, is there any way to deal with it，I don't understand the meaning of "just one more step" in your email

If my lysate contains 1 mM DTT, will it affect the experimental results? If so, is there any way to deal with it.

Hi, I was wondering if you could share with me the details of pre-blocking the beads: -What is the reagent used for pre-blocking? -If BSA is used, how is it immobilized on the surface of the bead?

Hello, I was wondering if you could tell me how the anti-HA antibody is immobilized on the beads? Is it a direct covalent bond or is there an "intermediate" antibody (e.g. Sheep anti-mouse IgG) through which the anti-HA is immobilized on the beads?

I wanted to check interaction between HA tagged protein and TAP fusion protein by fishing the HA tagged protein using HA- magnetic beads but I am worried that the protein A of the TAP fusion binds non specifically to the beads

1.What's the expiration date of the beads? 2.What' the binding capacity of the beads？ Thank you

What is the binding capacity of the beads?

What is the bead concentration?

Anti-HA Magnetic Beads

Anti-HA Magnetic Beads References

SAMPLE Certificate of Analysis (COA)

TEST CONDITIONS

How to request a Certificate of Analysis (COA) when it is not available online?

Anti-HA Magnetic Beads, 1 ml

Anti-HA Magnetic Beads, 5 ml

If my lysate contains 1MMDTT, will it affect the experimental results? If so, is there any way to deal with it，I don't understand the meaning of &quot;just one more step&quot; in your email

If my lysate contains 1 mM DTT, will it affect the experimental results? If so, is there any way to deal with it.

Hi, I was wondering if you could share with me the details of pre-blocking the beads: -What is the reagent used for pre-blocking? -If BSA is used, how is it immobilized on the surface of the bead?

Hello, I was wondering if you could tell me how the anti-HA antibody is immobilized on the beads? Is it a direct covalent bond or is there an &quot;intermediate&quot; antibody (e.g. Sheep anti-mouse IgG) through which the anti-HA is immobilized on the beads?

I wanted to check interaction between HA tagged protein and TAP fusion protein by fishing the HA tagged protein using HA- magnetic beads but I am worried that the protein A of the TAP fusion binds non specifically to the beads

1.What's the expiration date of the beads? 2.What' the binding capacity of the beads？ Thank you

What is the binding capacity of the beads?

What is the bead concentration?

If my lysate contains 1MMDTT, will it affect the experimental results? If so, is there any way to deal with it，I don't understand the meaning of "just one more step" in your email

Hello, I was wondering if you could tell me how the anti-HA antibody is immobilized on the beads? Is it a direct covalent bond or is there an "intermediate" antibody (e.g. Sheep anti-mouse IgG) through which the anti-HA is immobilized on the beads?