Date published: 2025-9-5

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Annexin V 抗体 (130): sc-32321

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说明书
  • Annexin V抗体(130)是小鼠单克隆IgG1 κ, 在10篇文献中引用,规格为200 µg/ml
  • 针对重组的禽类Annexin V
  • Annexin V 抗体 (130) 推荐用于WB 和 IP,检测human, avian and, to a lesser extent, mouse, 以及在较小程度上,检测 来源的 Annexin V
  • 抗Annexin V抗体(130)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Annexin V (130): sc-32321免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Annexin V Antibody (130) for WB applications. 的首选辅助检测试剂,这些试剂现与Annexin V Antibody (130) 打包提供(请参阅下面的订购信息)。

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関連項目

Annexin V抗体(130)是一种鼠单克隆IgG1 kappa轻链抗体,经过工程改造,可用于检测Annexin V(又称Lipocortin V或Annexin 5),可用于包括蛋白质印迹(WB)和免疫沉淀(IP)在内的各种应用。抗Annexin V抗体(130)针对重组禽Annexin V产生,对人和禽Annexin V有很强的反应性,同时对小鼠Annexin V也有一定程度的识别能力。Annexin V(130)抗体是钙结合蛋白家族中Annexin家族的重要成员,钙结合蛋白家族是离子通量调节、内吞作用、外排作用和细胞粘附等许多细胞过程所不可或缺的。Annexin V能够以钙依赖的方式结合磷脂,这对于细胞凋亡识别和细胞膜组织至关重要,因此Annexin V在细胞凋亡、炎症和癌症研究中扮演着重要角色。了解Annexin V的功能对于阐明其在调节蛋白激酶C(PKC)活性中的作用以及参与各种信号通路至关重要。Annexin V 单克隆抗体(130)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和几种 Alexa Fluor® 结合物,为不同的实验需求提供了多种选择。此外,Annexin V在组织和细胞中的广泛表达凸显了其在基本细胞过程中的重要性,以及作为各种疾病状态生物标志物的潜力。Anti-Annexin V抗体(130)为研究人员提供了强大的试剂,有助于深入研究这种重要蛋白的复杂作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Annexin V 抗体 (130)

sc-32321
200 µg/ml
$316.00

Annexin V (130): m-IgG Fc BP-HRP 套装

sc-526679
200 µg Ab; 10 µg BP
$354.00

Annexin V (130): m-IgG1 BP-HRP 套装

sc-532052
200 µg Ab; 20 µg BP
$354.00

Annexin V 抗体 (130) AC

sc-32321 AC
500 µg/ml, 25% agarose
$416.00

Annexin V 抗体 (130) HRP

sc-32321 HRP
200 µg/ml
$316.00

Annexin V 抗体 (130) FITC

sc-32321 FITC
200 µg/ml
$330.00

Annexin V 抗体 (130) PE

sc-32321 PE
200 µg/ml
$343.00

Annexin V 抗体 (130) Alexa Fluor® 488

sc-32321 AF488
200 µg/ml
$357.00

Annexin V 抗体 (130) Alexa Fluor® 546

sc-32321 AF546
200 µg/ml
$357.00

Annexin V 抗体 (130) Alexa Fluor® 594

sc-32321 AF594
200 µg/ml
$357.00

Annexin V 抗体 (130) Alexa Fluor® 647

sc-32321 AF647
200 µg/ml
$357.00

Annexin V 抗体 (130) Alexa Fluor® 680

sc-32321 AF680
200 µg/ml
$357.00

Annexin V 抗体 (130) Alexa Fluor® 790

sc-32321 AF790
200 µg/ml
$357.00

I will be conducting an immunofluorescence stain tomorrow with rat dopaminergic cells. What dilution factor should I use? 

Asked by: gatorgirl1
Thank you for your question. I would recommend a starting dilution of 1:50 for this antibody on dopaminergic cells. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-11-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Annexin V (130): sc-32321?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Works well in WB, but not with mouse tissuesThis annexin V antibody works well in WB with human liver. Strong signal and clean background. However, it does not recognize mouse Annexin V.
Date published: 2017-10-30
Rated 5 out of 5 by from Clear band in WB in nonClear band in WB in non-transfected 293T, human Annexin V transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Published data for WB in human lymph nodePublished data for WB in human lymph node containing breast carcinoma metastasis. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by from Published data for WB in mouse 3T3Published data for WB in mouse 3T3-L1 cells. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Published data for WB and IF in human transvenousPublished data for WB and IF in human transvenous endomyocardial sample. -SCBT Publication Review
Date published: 2015-02-15
Rated 5 out of 5 by from Published data for WB in chicken embryonicPublished data for WB in chicken embryonic trachea and kidney tissues. -SCBT Publication Review
Date published: 2015-02-08
Rated 5 out of 5 by from Strong positive band seen in WB in SLStrong positive band seen in WB in SL-29, HeLa and JAR whole cell lysates. -SCBT QC
Date published: 2014-01-28
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Annexin V 抗体 (130) is rated 4.9 out of 5 by 7.
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