Annexin I Antibody (EH17a): sc-12740

Annexin I Antibody (EH17a) is a mouse monoclonal IgG2b kappa light chain antibody specifically designed to detect Annexin I, also known by alternative names such as ANXA1, Lipocortin I, and Annexin A1. Annexin I mouse monoclonal antibody (EH17a) is raised against partially purified Annexin I derived from human polymorphonuclear leukocytes and demonstrates strong reactivity with Annexin I proteins from mouse, rat, and human sources, as well as additional species including bovine, across various applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Annexin I is a pivotal member of the annexin family of calcium-binding proteins, playing a crucial role in cellular processes like endocytosis, exocytosis, and cellular adhesion through its calcium-dependent phospholipid binding capabilities. The function of Annexin I is particularly important in mediating the anti-inflammatory responses elicited by glucocorticoids and in inhibiting cytosolic phospholipase A2 (cPLA2), a key enzyme involved in the inflammatory cascade. This makes Annexin I a significant target for research into inflammation, cancer, and various other disease mechanisms. Annexin I′s ability to regulate cellular trafficking and signal transduction underscores its importance in maintaining cellular homeostasis and responding to external stimuli. Annexin I monoclonal antibody (EH17a) is available in both non-conjugated form and multiple conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), agarose, and various Alexa Fluor® conjugates, providing versatility for a wide range of experimental setups. Annexin I monoclonal antibody (EH17a) is an essential tool for researchers aiming to elucidate the functional dynamics and regulatory mechanisms of Annexin I in diverse biological contexts, thereby advancing our understanding of its role in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Annexin I Antibody (EH17a) References:

1. Novel Role for the AnxA1-Fpr2/ALX Signaling Axis as a Key Regulator of Platelet Function to Promote Resolution of Inflammation.  |  Senchenkova, EY., et al. 2019. Circulation. 140: 319-335. PMID: 31154815
2. Anxa1 in smooth muscle cells protects against acute aortic dissection.  |  Zhou, C., et al. 2022. Cardiovasc Res. 118: 1564-1582. PMID: 33757117
3. ANXA1 Promotes Tumor Immune Evasion by Binding PARP1 and Upregulating Stat3-Induced Expression of PD-L1 in Multiple Cancers.  |  Xiao, D., et al. 2023. Cancer Immunol Res. 11: 1367-1383. PMID: 37566399
4. Tat-NTS peptide protects neurons against cerebral ischemia-reperfusion injury via ANXA1 SUMOylation in microglia.  |  Zhou, H., et al. 2023. Theranostics. 13: 5561-5583. PMID: 37908731
5. Modulation of annexin II tetramer by tyrosine phosphorylation.  |  Hubaishy, I., et al. 1995. Biochemistry. 34: 14527-34. PMID: 7578058
6. Structural evolution of the annexin supergene family.  |  Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
7. Annexin II up-regulates cellular levels of p11 protein by a post-translational mechanisms.  |  Puisieux, A., et al. 1996. Biochem J. 313 (Pt 1): 51-5. PMID: 8546709
8. Calcium-dependent binding of S100C to the N-terminal domain of annexin I.  |  Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
9. Annexin II tetramer: structure and function.  |  Waisman, DM. 1995. Mol Cell Biochem. 149-150: 301-22. PMID: 8569746
10. Dexamethasone induces an increase in intracellular and membrane-associated lipocortin-1 (annexin-1) in rat astrocyte primary cultures.  |  McLeod, JD. and Bolton, C. 1995. Cell Mol Neurobiol. 15: 193-205. PMID: 8590451
11. The concerted regulation of cPLA2, COX2, and lipocortin 1 expression by IL-1beta in A549 cells.  |  Croxtal, JD., et al. 1996. Biochem Biophys Res Commun. 220: 491-5. PMID: 8607793
12. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells.  |  Chasserot-Golaz, S., et al. 1996. J Cell Biol. 133: 1217-36. PMID: 8682860

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Annexin I Antibody (EH17a) has 50 citations in a variety of scientific publications.

Primary antibodies like Anti-Annexin I Antibody (EH17a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Annexin I Antibody (EH17a) Specifications

Alternate Product Name: Annexin I

Product Clone Name: EH17a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Annexin I

Epitope Species: human

Product Reactivity/Detection: Annexin I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 50 citations in various scientific publications

Available Conjugates/Forms for Annexin I Antibody (EH17a): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin