Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Amylase Double Nickase Plasmid (h): sc-400533-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Amylase 2a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Amylase 2a Double Nickase Plasmid (h) and Amylase 2a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AMY2A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Amylase Double Nickase Plasmid (h) | sc-400533-NIC | 20 µg | $410.00 | |||
Amylase 2a Double Nickase Plasmid (h2) | sc-400533-NIC-2 | 20 µg | $410.00 |
AMY2A encodes pancreatic α-amylase, a secreted glycoside hydrolase that initiates dietary starch digestion by cleaving internal α-1,4-glycosidic bonds to generate maltose and oligosaccharides. Its expression is tightly linked to acinar cell identity and regulated secretory programs, integrating with exocrine pancreas pathways that support nutrient processing and digestive enzyme homeostasis. AMY2A is commonly used as a functional marker of pancreatic exocrine differentiation and enzyme output in cellular models and organoids. Altered amylase abundance or activity is frequently evaluated in studies of pancreatic stress and inflammatory states, as well as metabolic contexts that influence digestive capacity.
Amylase 2a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AMY2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AMY2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AMY2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AMY2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.